AlphaFold2 Can Predict Single-Mutation Effects

  • John M. Mcbride
  • , Konstantin Polev
  • , Amirbek Abdirasulov
  • , Vladimir Reinharz
  • , Bartosz A. Grzybowski
  • , Tsvi Tlusty

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

AlphaFold2 (AF) is a promising tool, but is it accurate enough to predict single mutation effects Here, we report that the localized structural deformation between protein pairs differing by only 1-3 mutations - as measured by the effective strain - is correlated across 3901 experimental and AF-predicted structures. Furthermore, analysis of ∼11 000 proteins shows that the local structural change correlates with various phenotypic changes. These findings suggest that AF can predict the range and magnitude of single-mutation effects on average, and we propose a method to improve precision of AF predictions and to indicate when predictions are unreliable.

Original languageEnglish
Article number218401
JournalPhysical Review Letters
Volume131
Issue number21
DOIs
StatePublished - 24 Nov 2023
Externally publishedYes

ASJC Scopus subject areas

  • General Physics and Astronomy

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