Amniotic fluid soluble human leukocyte antigen-G in term and preterm parturition, and intra-amniotic infection/inflammation

Juan Pedro Kusanovic, Roberto Romero, Cristiano Jodicke, Shali Mazaki-Tovi, Edi Vaisbuch, Offer Erez, Pooja Mittal, Francesca Gotsch, Tinnakorn Chaiworapongsa, Sam S. Edwin, Percy Pacora, Sonia S. Hassan

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Objective. Circulating soluble human leukocyte antigen-G (sHLA-G) has been associated with pregnancy complications, and determination of sHLA-G concentrations in amniotic fluid (AF) has been reported in normal pregnancies. Our aim was to determine if the AF concentrations of sHLA-G change with advancing gestation, spontaneous labor at term, and in patients with spontaneous preterm labor (PTL) with intact membranes, as well as in those with preterm prelabor rupture of membranes (PROM), in the presence or absence of intra-amniotic infection/inflammation (IAI). Study design. This cross-sectional study included the following groups: (1) mid-trimester (n=55); (2) normal pregnancy at term with (n=50) and without (n=50) labor; (3) spontaneous PTL with intact membranes divided into: (a) PTL who delivered at term (n=153); (b) PTL who delivered preterm without IAI (n=108); and (c) PTL with IAI (n=84); and (4) preterm PROM with (n=46) and without (n=44) IAI. sHLA-G concentrations were determined by ELISA. Nonparametric statistics were used for analysis. Results. (1) Among patients with PTL, the median AF sHLA-G concentration was higher in patients with IAI than in those without IAI or women that delivered at term (p<0.001 for both comparisons); (2) Similarly, patients with preterm PROM and IAI had higher median AF sHLA-G concentrations than those without IAI (p=0.004); (3) Among patients with PTL and delivery, those with histologic chorioamnionitis and/or funisitis had a higher median AF sHLA-G concentration than those without histologic inflammation (p<0.001); and (4) The median AF sHLA-G concentration did not change with advancing gestational age. Conclusions. AF sHLA-G concentrations are elevated in preterm parturition associated to IAI as well as in histologic chorioamnionitis. We propose that sHLA-G may participate in the regulation of the host immune response against intraamniotic infection.

Original languageEnglish
Pages (from-to)1151-1166
Number of pages16
JournalJournal of Maternal-Fetal and Neonatal Medicine
Issue number12
StatePublished - 1 Dec 2009
Externally publishedYes


  • Amniocentesis
  • Chorioamnionitis
  • HLA-G
  • Microbial invasion of the amniotic cavity
  • Pregnancy
  • Preterm delivery
  • Preterm labor
  • Preterm prelabor rupture of membranes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology


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