Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells

Hemant P. Borase, Chandrashekhar D. Patil, Ismael P. Sauter, Marilise B. Rott, Satish V. Patil

Research output: Contribution to journalLetterpeer-review

36 Scopus citations

Abstract

Acanthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. Jatropha curcas, Jatropha gossypifolia and Euphorbia milii plant extracts synthesized stable silver nanoparticles (AgNPs) that were relatively stable. Amoebicidal activity of J. gossypifolia, J. curcas and E. milii leaf extracts showed little effect on viability of Acanthamoeba castellanii trophozoites. Plant-synthesized AgNPs showed higher amoebicidal activity. AgNPs synthesized by J. gossypifolia extract were able to kill 74-27% of the trophozoites at concentrations of 25-1.56 μg mL-1. AgNPs were nontoxic at minimum inhibitory concentration with peripheral blood mononuclear cells. These results suggest biologically synthesized nanoparticles as an alternative candidate for treatment of Acanthamoeba infections.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalFEMS Microbiology Letters
Volume345
Issue number2
DOIs
StatePublished - 1 Aug 2013
Externally publishedYes

Keywords

  • Acanthamoeba
  • Amoebicidal
  • Cytotoxicity
  • Plant extract
  • Silver nanoparticles

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells'. Together they form a unique fingerprint.

Cite this