Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells

Hemant P. Borase; Chandrashekhar D. Patil; Ismael P. Sauter; Marilise B. Rott; Satish V. Patil

doi:10.1111/1574-6968.12195

Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells

Hemant P. Borase, Chandrashekhar D. Patil, Ismael P. Sauter, Marilise B. Rott, Satish V. Patil

Research output: Contribution to journal › Letter › peer-review

36 Scopus citations

Abstract

Acanthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. Jatropha curcas, Jatropha gossypifolia and Euphorbia milii plant extracts synthesized stable silver nanoparticles (AgNPs) that were relatively stable. Amoebicidal activity of J. gossypifolia, J. curcas and E. milii leaf extracts showed little effect on viability of Acanthamoeba castellanii trophozoites. Plant-synthesized AgNPs showed higher amoebicidal activity. AgNPs synthesized by J. gossypifolia extract were able to kill 74-27% of the trophozoites at concentrations of 25-1.56 μg mL^-1. AgNPs were nontoxic at minimum inhibitory concentration with peripheral blood mononuclear cells. These results suggest biologically synthesized nanoparticles as an alternative candidate for treatment of Acanthamoeba infections.

Original language	English
Pages (from-to)	127-131
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	345
Issue number	2
DOIs	https://doi.org/10.1111/1574-6968.12195
State	Published - 1 Aug 2013
Externally published	Yes

Keywords

Acanthamoeba
Amoebicidal
Cytotoxicity
Plant extract
Silver nanoparticles

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/1574-6968.12195

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title = "Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells",

abstract = "Acanthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. Jatropha curcas, Jatropha gossypifolia and Euphorbia milii plant extracts synthesized stable silver nanoparticles (AgNPs) that were relatively stable. Amoebicidal activity of J. gossypifolia, J. curcas and E. milii leaf extracts showed little effect on viability of Acanthamoeba castellanii trophozoites. Plant-synthesized AgNPs showed higher amoebicidal activity. AgNPs synthesized by J. gossypifolia extract were able to kill 74-27% of the trophozoites at concentrations of 25-1.56 μg mL-1. AgNPs were nontoxic at minimum inhibitory concentration with peripheral blood mononuclear cells. These results suggest biologically synthesized nanoparticles as an alternative candidate for treatment of Acanthamoeba infections.",

keywords = "Acanthamoeba, Amoebicidal, Cytotoxicity, Plant extract, Silver nanoparticles",

author = "Borase, {Hemant P.} and Patil, {Chandrashekhar D.} and Sauter, {Ismael P.} and Rott, {Marilise B.} and Patil, {Satish V.}",

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doi = "10.1111/1574-6968.12195",

language = "English",

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journal = "FEMS Microbiology Letters",

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T1 - Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells

AU - Borase, Hemant P.

AU - Patil, Chandrashekhar D.

AU - Sauter, Ismael P.

AU - Rott, Marilise B.

AU - Patil, Satish V.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Acanthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. Jatropha curcas, Jatropha gossypifolia and Euphorbia milii plant extracts synthesized stable silver nanoparticles (AgNPs) that were relatively stable. Amoebicidal activity of J. gossypifolia, J. curcas and E. milii leaf extracts showed little effect on viability of Acanthamoeba castellanii trophozoites. Plant-synthesized AgNPs showed higher amoebicidal activity. AgNPs synthesized by J. gossypifolia extract were able to kill 74-27% of the trophozoites at concentrations of 25-1.56 μg mL-1. AgNPs were nontoxic at minimum inhibitory concentration with peripheral blood mononuclear cells. These results suggest biologically synthesized nanoparticles as an alternative candidate for treatment of Acanthamoeba infections.

AB - Acanthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. Jatropha curcas, Jatropha gossypifolia and Euphorbia milii plant extracts synthesized stable silver nanoparticles (AgNPs) that were relatively stable. Amoebicidal activity of J. gossypifolia, J. curcas and E. milii leaf extracts showed little effect on viability of Acanthamoeba castellanii trophozoites. Plant-synthesized AgNPs showed higher amoebicidal activity. AgNPs synthesized by J. gossypifolia extract were able to kill 74-27% of the trophozoites at concentrations of 25-1.56 μg mL-1. AgNPs were nontoxic at minimum inhibitory concentration with peripheral blood mononuclear cells. These results suggest biologically synthesized nanoparticles as an alternative candidate for treatment of Acanthamoeba infections.

KW - Acanthamoeba

KW - Amoebicidal

KW - Cytotoxicity

KW - Plant extract

KW - Silver nanoparticles

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U2 - 10.1111/1574-6968.12195

DO - 10.1111/1574-6968.12195

M3 - Letter

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AN - SCOPUS:84880578088

VL - 345

SP - 127

EP - 131

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -

Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells

Abstract

Keywords

ASJC Scopus subject areas

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