Amperometric biosensor based on the electro-copolymerization of a conductive biotinylated-pyrrole and alginate-pyrrole

Iulia I. Niţǎ, Khalil Abu-Rabeah, Anca M. Tencaliec, Serge Cosnier, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A new matrix was designed and produced, which entailed the co-electropolymerization of pyrrole-biotin (Py-B) with pyrrole-alginate (Alg-Py). The aim was to demonstrate that improved performance could be obtained in a classical model system such as monitoring for the presence of glucose. The sensor construction consisted of the conjugation of biotinylated-glucose oxidase (B-GOx) to B-Py through avidin (Av) bridges, followed by copolymerization with Alg-Py. When the aforementioned set-up did not include the pyrrole-modified alginate but unmodified alginate, its performance values were significantly less. During measurement of the concentration of the model glucose analyte via hydrogen peroxide oxidation, the current was observed to be conducted faster to the electrode surface, therefore providing a lower response time, and a higher sensitivity value, using a relatively low enzyme load.

Original languageEnglish
Pages (from-to)1117-1122
Number of pages6
JournalSynthetic Metals
Volume159
Issue number12
DOIs
StatePublished - 1 Jan 2009

Keywords

  • Alg-Py
  • B-Gox
  • Biosensor
  • Copolymerization
  • Py-B

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