Amperometric immunosensor for the detection of anti-West Nile virus IgG

Rodica E. Ionescu, Serge Cosnier, Sebastien Herrmann, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

An amperometric immunosensor for the detection of West Nile virus (WNV) IgG was developed. This device was based on the immobilization of T7 phages, which were modified by an additional peptide sequence taken from the virus and used as antigen. The electropolymerization of a phage-amphiphilic pyrrole ammonium mixture previously adsorbed on the electrode surface provided an efficient entrapment of phages in a polypyrrole film. After incubation with a secondary peroxidase-labeled antibody, the immunosensors were applied to the quantitative amperometric determination of WNV-antibody at 0 V vs Ag/AgCl via the reduction of the enzymically generated quinone in the presence of hydroquinone and H 2O2. The optimum immunosensor configuration detected low WNV-antibody dilutions down to a titer of 1:107 with an excellent regeneration of the immunosensor response by glycine treatment.

Original languageEnglish
Pages (from-to)8662-8668
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number22
DOIs
StatePublished - 15 Nov 2007

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint

Dive into the research topics of 'Amperometric immunosensor for the detection of anti-West Nile virus IgG'. Together they form a unique fingerprint.

Cite this