Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer

Rodica E. Ionescu; Serge Cosnier; Gregoire Herzog; Karine Gorgy; Boaz Leshem; Sebastien Herrmann; Robert S. Marks

doi:10.1016/j.enzmictec.2006.07.010

Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer

Rodica E. Ionescu, Serge Cosnier, Gregoire Herzog, Karine Gorgy, Boaz Leshem, Sebastien Herrmann, Robert S. Marks

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A polymeric film consisting of two distinct monomers such as pyrrole-benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV-antibody dilutions ranging from 10 to 10⁶ was created with a relatively short response time of 30 s after substrate injection.

Original language	English
Pages (from-to)	403-408
Number of pages	6
Journal	Enzyme and Microbial Technology
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/j.enzmictec.2006.07.010
State	Published - 5 Feb 2007

Keywords

Bacteriophage
Benzophenone
Immunosensor
Polypyrrole
West Nile virus antibodies

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.enzmictec.2006.07.010

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title = "Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer",

abstract = "A polymeric film consisting of two distinct monomers such as pyrrole-benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV-antibody dilutions ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection.",

keywords = "Bacteriophage, Benzophenone, Immunosensor, Polypyrrole, West Nile virus antibodies",

author = "Ionescu, {Rodica E.} and Serge Cosnier and Gregoire Herzog and Karine Gorgy and Boaz Leshem and Sebastien Herrmann and Marks, {Robert S.}",

note = "Funding Information: EU is thanked for funding under the 6th Framework contract: NMP-A-CT-2003-505485-1. Gregoire Herzog is grateful to the CNRS for postdoctoral fellowship. The sequence of the putative WNV epitope was generously provided by the Pr. Bracha Rager-Zisman (Department of Microbiology and Immunology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel). Rodica Ionescu thanks the French Ministry of Foreign Affairs for a Postdoctoral Chateaubriand fellowship. ",

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doi = "10.1016/j.enzmictec.2006.07.010",

language = "English",

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pages = "403--408",

journal = "Enzyme and Microbial Technology",

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T1 - Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer

AU - Ionescu, Rodica E.

AU - Cosnier, Serge

AU - Herzog, Gregoire

AU - Gorgy, Karine

AU - Leshem, Boaz

AU - Herrmann, Sebastien

AU - Marks, Robert S.

N1 - Funding Information: EU is thanked for funding under the 6th Framework contract: NMP-A-CT-2003-505485-1. Gregoire Herzog is grateful to the CNRS for postdoctoral fellowship. The sequence of the putative WNV epitope was generously provided by the Pr. Bracha Rager-Zisman (Department of Microbiology and Immunology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel). Rodica Ionescu thanks the French Ministry of Foreign Affairs for a Postdoctoral Chateaubriand fellowship.

PY - 2007/2/5

Y1 - 2007/2/5

N2 - A polymeric film consisting of two distinct monomers such as pyrrole-benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV-antibody dilutions ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection.

AB - A polymeric film consisting of two distinct monomers such as pyrrole-benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV-antibody dilutions ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection.

KW - Bacteriophage

KW - Benzophenone

KW - Immunosensor

KW - Polypyrrole

KW - West Nile virus antibodies

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U2 - 10.1016/j.enzmictec.2006.07.010

DO - 10.1016/j.enzmictec.2006.07.010

M3 - Article

AN - SCOPUS:33846196163

SN - 0141-0229

VL - 40

SP - 403

EP - 408

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 3

ER -

Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer

Abstract

Keywords

ASJC Scopus subject areas

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