Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles

Y. Weizmann, F. Patolsky, I. Willner

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

A novel amplification route for DNA detection based on the deposition of gold on a 10 nm Au-colloid/avidin conjugate label acting as a 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce the catalyzed deposition of gold on the piezoelectric crystals, Three different DNA detection schemes are described: (i) analysis of a 27-base nucleic acid fragment; (ii) analysis of the entire M13φ DNA (7229 bases); and (iii) detection of a single-base mismatch in a DNA. Ultrasensitive detection of DNA is accomplished by the catalyzed deposition of gold, detection limit ∼ 1 × 10-15 M.

Original languageEnglish
Pages (from-to)1502-1504
Number of pages3
JournalThe Analyst
Volume126
Issue number9
DOIs
StatePublished - 1 Jan 2001
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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