Amplified telomerase analysis by using rotating magnetic particles: The rapid and sensitive detection of cancer cells

Yossi Weizmann, Fernando Patolsky, Eugenii Katz, Itamar Willner

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A highly sensitive telomerase detection method that involves amplified telomerase analysis and the use of rotating magnetic particles has been developed. Magnetic particles, functionalized with a primer (1) that is recognized by telomerase, are mixed with a nucleotide mixture that includes biotinylated-dUTP, and telomerase-induced elongation of the primers proceeds with simultaneous biotin incorporation. Avidin-Horseradish peroxidase conjugate, coupled to biotin labels, yields the biocatalytic functional particles. Mixing the resulting particles with naphthoquinone-modified magnetic particles enables the optoelectronic detection of telomerase. Attraction of the magnetic particles to an electrode, followed by rotation of the particles, causes the electrocatalytic reduction of O2 to H2O2 and HRP-catalyzed oxidation of luminol (3); this results in chemilumunescence. The intensity of the emitted light depends on the telomerase content of the sample and the rotation speed of the particles. A minimum number of 10 cancer cells could be detected.

Original languageEnglish
Pages (from-to)943-948
Number of pages6
JournalChemBioChem
Volume5
Issue number7
DOIs
StatePublished - 5 Jul 2004
Externally publishedYes

Keywords

  • Biosensors
  • Cancer
  • DNA
  • Enzymes
  • Telomerase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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