Amyloid fishing: β-Amyloid adsorption using tailor-made coated titania nanoparticles

Elad Arad; Raz Jelinek; Hanna Rapaport

doi:10.1016/j.colsurfb.2022.112374

Amyloid fishing: β-Amyloid adsorption using tailor-made coated titania nanoparticles

Elad Arad, Raz Jelinek, Hanna Rapaport

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Amyloidoses are a family of diseases characterized by abnormal protein folding that leads to fibril aggregates, amyloids. Extensive research efforts are devoted to developing inhibitors to amyloid aggregates. Here we set to explore functionalized titania (TiO₂) nanoparticles (NPs) as potential amyloid inhibiting agents. TiO₂ NPs were coated by a catechol derivative, dihydroxy-phenylalanine propanoic acid (DPA), and further conjugated to the amyloids' specific dye Congo-Red (CR). TiO₂-DPA-CR NPs were found to target mature fibrils of β-amyloid (Aβ). Moreover, coated NPs incubated with Aβ proteins suppressed amyloid fibrillation. TiO₂-DPA-CR were found to target amyloids in solution and induce their sedimentation upon centrifugation. This work demonstrates the potential utilization of TiO₂-DPA NPs for labeling and facilely separating from solution mature amyloid fibrils.

Original language	English
Article number	112374
Journal	Colloids and Surfaces B: Biointerfaces
Volume	212
DOIs	https://doi.org/10.1016/j.colsurfb.2022.112374
State	Published - 1 Apr 2022

Keywords

Beta-amyloid
Catechol-coating
Congo-red
Titania
Titanium-dioxide

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.colsurfb.2022.112374

Cite this

@article{3f4a86fa9fb949ec8150a9bdddf84a53,

title = "Amyloid fishing: β-Amyloid adsorption using tailor-made coated titania nanoparticles",

abstract = "Amyloidoses are a family of diseases characterized by abnormal protein folding that leads to fibril aggregates, amyloids. Extensive research efforts are devoted to developing inhibitors to amyloid aggregates. Here we set to explore functionalized titania (TiO2) nanoparticles (NPs) as potential amyloid inhibiting agents. TiO2 NPs were coated by a catechol derivative, dihydroxy-phenylalanine propanoic acid (DPA), and further conjugated to the amyloids' specific dye Congo-Red (CR). TiO2-DPA-CR NPs were found to target mature fibrils of β-amyloid (Aβ). Moreover, coated NPs incubated with Aβ proteins suppressed amyloid fibrillation. TiO2-DPA-CR were found to target amyloids in solution and induce their sedimentation upon centrifugation. This work demonstrates the potential utilization of TiO2-DPA NPs for labeling and facilely separating from solution mature amyloid fibrils.",

keywords = "Beta-amyloid, Catechol-coating, Congo-red, Titania, Titanium-dioxide",

author = "Elad Arad and Raz Jelinek and Hanna Rapaport",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = apr,

day = "1",

doi = "10.1016/j.colsurfb.2022.112374",

language = "English",

volume = "212",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

}

TY - JOUR

T1 - Amyloid fishing

T2 - β-Amyloid adsorption using tailor-made coated titania nanoparticles

AU - Arad, Elad

AU - Jelinek, Raz

AU - Rapaport, Hanna

PY - 2022/4/1

Y1 - 2022/4/1

N2 - Amyloidoses are a family of diseases characterized by abnormal protein folding that leads to fibril aggregates, amyloids. Extensive research efforts are devoted to developing inhibitors to amyloid aggregates. Here we set to explore functionalized titania (TiO2) nanoparticles (NPs) as potential amyloid inhibiting agents. TiO2 NPs were coated by a catechol derivative, dihydroxy-phenylalanine propanoic acid (DPA), and further conjugated to the amyloids' specific dye Congo-Red (CR). TiO2-DPA-CR NPs were found to target mature fibrils of β-amyloid (Aβ). Moreover, coated NPs incubated with Aβ proteins suppressed amyloid fibrillation. TiO2-DPA-CR were found to target amyloids in solution and induce their sedimentation upon centrifugation. This work demonstrates the potential utilization of TiO2-DPA NPs for labeling and facilely separating from solution mature amyloid fibrils.

AB - Amyloidoses are a family of diseases characterized by abnormal protein folding that leads to fibril aggregates, amyloids. Extensive research efforts are devoted to developing inhibitors to amyloid aggregates. Here we set to explore functionalized titania (TiO2) nanoparticles (NPs) as potential amyloid inhibiting agents. TiO2 NPs were coated by a catechol derivative, dihydroxy-phenylalanine propanoic acid (DPA), and further conjugated to the amyloids' specific dye Congo-Red (CR). TiO2-DPA-CR NPs were found to target mature fibrils of β-amyloid (Aβ). Moreover, coated NPs incubated with Aβ proteins suppressed amyloid fibrillation. TiO2-DPA-CR were found to target amyloids in solution and induce their sedimentation upon centrifugation. This work demonstrates the potential utilization of TiO2-DPA NPs for labeling and facilely separating from solution mature amyloid fibrils.

KW - Beta-amyloid

KW - Catechol-coating

KW - Congo-red

KW - Titania

KW - Titanium-dioxide

UR - http://www.scopus.com/inward/record.url?scp=85123793562&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2022.112374

DO - 10.1016/j.colsurfb.2022.112374

M3 - Article

C2 - 35121429

AN - SCOPUS:85123793562

SN - 0927-7765

VL - 212

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

M1 - 112374

ER -

Amyloid fishing: β-Amyloid adsorption using tailor-made coated titania nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this