Amyloid precursor protein secretion via muscarinic receptors: Reduced desensitization using the m1-selective agonist AF102B

Rachel Haring; David Gurwitz; Jacob Barg; Ronit Pinkas-Kramarski; Eliahu Heldman; Zipora Pittel; Ada Wengier; Haim Meshulam; Daniele Marciano; Yishai Karton; Abraham Fisher

doi:10.1006/bbrc.1994.2232

Amyloid precursor protein secretion via muscarinic receptors: Reduced desensitization using the m1-selective agonist AF102B

Rachel Haring, David Gurwitz, Jacob Barg, Ronit Pinkas-Kramarski, Eliahu Heldman, Zipora Pittel, Ada Wengier, Haim Meshulam, Daniele Marciano, Yishai Karton, Abraham Fisher

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Secretion of amyloid precursor protein (APP) by cultured cells is coupled to several receptors, including m1 muscarinic (m1AChR), and is associated with decreased production of βA4 amyloid. Secreted and cell-associated APP levels were measured in m1AChR-transfected PC12 cells stimulated with the non-selective agonist carbachol or the M1-selective agonist, AF102B. Secreted APP levels following stimulation with AF102B (5-60 min) were about half compared with carbachol. Yet, following 24 h stimulation with carbachol or AF102B, cell-associated APP levels were similarly decreased. This may be associated with a smaller reduction in APP secretion following 24 h stimulation with AF102B as compared with carbachol. AF102B may therefore have an advangage over non-selective muscarinic ligands for sustained decrease of cell-associated APP.

Original language	English
Pages (from-to)	652-658
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	203
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1994.2232
State	Published - 30 Aug 1994
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Haring, R., Gurwitz, D., Barg, J., Pinkas-Kramarski, R., Heldman, E., Pittel, Z., Wengier, A., Meshulam, H., Marciano, D., Karton, Y., & Fisher, A. (1994). Amyloid precursor protein secretion via muscarinic receptors: Reduced desensitization using the m1-selective agonist AF102B. Biochemical and Biophysical Research Communications, 203(1), 652-658. https://doi.org/10.1006/bbrc.1994.2232

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title = "Amyloid precursor protein secretion via muscarinic receptors: Reduced desensitization using the m1-selective agonist AF102B",

abstract = "Secretion of amyloid precursor protein (APP) by cultured cells is coupled to several receptors, including m1 muscarinic (m1AChR), and is associated with decreased production of βA4 amyloid. Secreted and cell-associated APP levels were measured in m1AChR-transfected PC12 cells stimulated with the non-selective agonist carbachol or the M1-selective agonist, AF102B. Secreted APP levels following stimulation with AF102B (5-60 min) were about half compared with carbachol. Yet, following 24 h stimulation with carbachol or AF102B, cell-associated APP levels were similarly decreased. This may be associated with a smaller reduction in APP secretion following 24 h stimulation with AF102B as compared with carbachol. AF102B may therefore have an advangage over non-selective muscarinic ligands for sustained decrease of cell-associated APP.",

author = "Rachel Haring and David Gurwitz and Jacob Barg and Ronit Pinkas-Kramarski and Eliahu Heldman and Zipora Pittel and Ada Wengier and Haim Meshulam and Daniele Marciano and Yishai Karton and Abraham Fisher",

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Haring, R, Gurwitz, D, Barg, J, Pinkas-Kramarski, R, Heldman, E, Pittel, Z, Wengier, A, Meshulam, H, Marciano, D, Karton, Y & Fisher, A 1994, 'Amyloid precursor protein secretion via muscarinic receptors: Reduced desensitization using the m1-selective agonist AF102B', Biochemical and Biophysical Research Communications, vol. 203, no. 1, pp. 652-658. https://doi.org/10.1006/bbrc.1994.2232

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T1 - Amyloid precursor protein secretion via muscarinic receptors

T2 - Reduced desensitization using the m1-selective agonist AF102B

AU - Haring, Rachel

AU - Gurwitz, David

AU - Barg, Jacob

AU - Pinkas-Kramarski, Ronit

AU - Heldman, Eliahu

AU - Pittel, Zipora

AU - Wengier, Ada

AU - Meshulam, Haim

AU - Marciano, Daniele

AU - Karton, Yishai

AU - Fisher, Abraham

PY - 1994/8/30

Y1 - 1994/8/30

N2 - Secretion of amyloid precursor protein (APP) by cultured cells is coupled to several receptors, including m1 muscarinic (m1AChR), and is associated with decreased production of βA4 amyloid. Secreted and cell-associated APP levels were measured in m1AChR-transfected PC12 cells stimulated with the non-selective agonist carbachol or the M1-selective agonist, AF102B. Secreted APP levels following stimulation with AF102B (5-60 min) were about half compared with carbachol. Yet, following 24 h stimulation with carbachol or AF102B, cell-associated APP levels were similarly decreased. This may be associated with a smaller reduction in APP secretion following 24 h stimulation with AF102B as compared with carbachol. AF102B may therefore have an advangage over non-selective muscarinic ligands for sustained decrease of cell-associated APP.

AB - Secretion of amyloid precursor protein (APP) by cultured cells is coupled to several receptors, including m1 muscarinic (m1AChR), and is associated with decreased production of βA4 amyloid. Secreted and cell-associated APP levels were measured in m1AChR-transfected PC12 cells stimulated with the non-selective agonist carbachol or the M1-selective agonist, AF102B. Secreted APP levels following stimulation with AF102B (5-60 min) were about half compared with carbachol. Yet, following 24 h stimulation with carbachol or AF102B, cell-associated APP levels were similarly decreased. This may be associated with a smaller reduction in APP secretion following 24 h stimulation with AF102B as compared with carbachol. AF102B may therefore have an advangage over non-selective muscarinic ligands for sustained decrease of cell-associated APP.

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Amyloid precursor protein secretion via muscarinic receptors: Reduced desensitization using the m1-selective agonist AF102B

Abstract

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