An Alternate Method for Staining Plastic Embedded Autoradiographs

Janette M. Krum; Jefferey M. Rosenstein; William F. Silverman

doi:10.1179/his.1991.14.1.43

An Alternate Method for Staining Plastic Embedded Autoradiographs

Janette M. Krum, Jefferey M. Rosenstein, William F. Silverman

Department of Physiology and Cell Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have developed a rapid and reliable method for staining plastic embedded autoradiographs for in situ labeling protocols that eliminates several problems sometimes encountered with other methods, such as displacement of silver grains and uptake of stain by the emulsion layer itself. The procedure features the brief (less than one minute) application of toluidine blue to plastic embedded autoradiographs with moderate warming. The quality of staining obtained by this method is both consistent and flexible, allowing varying degrees of intensification without the danger of damaging the emulsion layer. The principle advantages of this procedure over other techniques to stain plastic embedded autoradiographs are its simplicity, rapidity and reproducibility. (The J Histotechnol 14:43, 1991)

Original language	English
Pages (from-to)	43-45
Number of pages	3
Journal	Journal of Histotechnology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1179/his.1991.14.1.43
State	Published - 1 Jan 1991

Keywords

Autoradiography
In situ labeling
Plastic embedding

ASJC Scopus subject areas

Anatomy
Histology
Medical Laboratory Technology

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10.1179/his.1991.14.1.43

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title = "An Alternate Method for Staining Plastic Embedded Autoradiographs",

abstract = "We have developed a rapid and reliable method for staining plastic embedded autoradiographs for in situ labeling protocols that eliminates several problems sometimes encountered with other methods, such as displacement of silver grains and uptake of stain by the emulsion layer itself. The procedure features the brief (less than one minute) application of toluidine blue to plastic embedded autoradiographs with moderate warming. The quality of staining obtained by this method is both consistent and flexible, allowing varying degrees of intensification without the danger of damaging the emulsion layer. The principle advantages of this procedure over other techniques to stain plastic embedded autoradiographs are its simplicity, rapidity and reproducibility. (The J Histotechnol 14:43, 1991)",

keywords = "Autoradiography, In situ labeling, Plastic embedding",

author = "Krum, {Janette M.} and Rosenstein, {Jefferey M.} and Silverman, {William F.}",

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AU - Rosenstein, Jefferey M.

AU - Silverman, William F.

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N2 - We have developed a rapid and reliable method for staining plastic embedded autoradiographs for in situ labeling protocols that eliminates several problems sometimes encountered with other methods, such as displacement of silver grains and uptake of stain by the emulsion layer itself. The procedure features the brief (less than one minute) application of toluidine blue to plastic embedded autoradiographs with moderate warming. The quality of staining obtained by this method is both consistent and flexible, allowing varying degrees of intensification without the danger of damaging the emulsion layer. The principle advantages of this procedure over other techniques to stain plastic embedded autoradiographs are its simplicity, rapidity and reproducibility. (The J Histotechnol 14:43, 1991)

AB - We have developed a rapid and reliable method for staining plastic embedded autoradiographs for in situ labeling protocols that eliminates several problems sometimes encountered with other methods, such as displacement of silver grains and uptake of stain by the emulsion layer itself. The procedure features the brief (less than one minute) application of toluidine blue to plastic embedded autoradiographs with moderate warming. The quality of staining obtained by this method is both consistent and flexible, allowing varying degrees of intensification without the danger of damaging the emulsion layer. The principle advantages of this procedure over other techniques to stain plastic embedded autoradiographs are its simplicity, rapidity and reproducibility. (The J Histotechnol 14:43, 1991)

KW - Autoradiography

KW - In situ labeling

KW - Plastic embedding

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An Alternate Method for Staining Plastic Embedded Autoradiographs

Abstract

Keywords

ASJC Scopus subject areas

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