An Alternative Model of Laser-Induced Stroke in the Motor Cortex of Rats

Matthew Boyko, Ruslan Kuts, Benjamin F. Gruenbaum, Philip Tsenter, Julia Grinshpun, Dmitry Frank, Vladislav Zvenigorodsky, Israel Melamed, Evgeni Brotfain, Alexander Zlotnik

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: A common experimental rodent model for stroke includes induction by a technique in which middle cerebral artery is transiently (MCAO-t) or permanently (MCAO-p) occluded by catheterization. However, this model has prominent disadvantages which consist of the high variability of localization and size of the ischemic area, cases of intracranial hemorrhage and high mortality. Furthermore, the duration of a single MCAO operation takes about thirty minutes and requires highly trained staff. In this article, we propose an alternative method, which is based on laser-induced stroke in the motor cortex. In our research, we compared the original MCAO-p and MCAO-t models and a novel laser model. Results: Compared with the impact of original MCAO-p and MCAO-t technique on brain tissue, the minimally invasive laser model demonstrated a decrease in: variability in body temperature, percent of infarcted volume, blood brain barrier breakdown and brain edema, as well as a prominent decrease of mortality and intracranial hemorrhage. Among other findings of this article, it can be noted that damage to the brain tissue in laser groups occurred only in the region of the motor cortex, without involving the striatal area. Conclusions: The data presented in this paper show that the model of laser irradiation can serve as an effective method of inducible brain cortical infarction and may lead to a better understanding of the pathophysiology of ischemic stroke and the future development of new drugs and other neuro-protective agents.

Original languageEnglish
Article number9
JournalBiological Procedures Online
Volume21
Issue number1
DOIs
StatePublished - 16 May 2019
Externally publishedYes

Keywords

  • Cortex
  • Laser-technique
  • MCAO
  • Outcome
  • Stroke
  • Variability
  • model

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)

Fingerprint

Dive into the research topics of 'An Alternative Model of Laser-Induced Stroke in the Motor Cortex of Rats'. Together they form a unique fingerprint.

Cite this