An extension of the Minute Virus of Mice tissue tropism

Igor Etingov, Refael Itah, Michal Mincberg, Ayelet Keren-Naus, Hyun Joo Nam, Mavis Agbandje-McKenna, Claytus Davis

    Research output: Contribution to journalArticlepeer-review

    5 Scopus citations

    Abstract

    Well-defined tissue tropism makes Autonomous Parvoviruses a valuable model for studies of virus-cell interactions and gene therapy research. We developed a new Minute Virus of Mice variant, different from the known prototype (MVMp) and immunosuppressive (MVMi) strains. The new virus variant, designated F1, was isolated from the culture of semi-permissive Fisher Rat Fibroblasts, F111, infected with MVMp. The F1 genome carried point mutations in regions known to determine the mutually restricted host ranges of MVMp and MVMi. In F111 cells, F1 cytotoxicity, gene expression and multiplication were significantly higher compared to MVMp. Conversely the wild-type virus propagated in MVMp-permissive cells more efficiently than the F1. Reversion of the F1-specific mutations to wild-type MVMp sequence, following reverse-passaging of the mutant virus in MVMp-permissive cells, confirmed a specific adaptation of the F1 virus to F111 cells. Considerable divergence in tissue specificities between the wild-type and mutant viruses was demonstrated in vivo.

    Original languageEnglish
    Pages (from-to)245-255
    Number of pages11
    JournalVirology
    Volume379
    Issue number2
    DOIs
    StatePublished - 30 Sep 2008

    Keywords

    • Adaptation
    • Host range
    • MVM
    • Minute Virus of Mice
    • Parvovirus
    • Quasispecies

    ASJC Scopus subject areas

    • Virology

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