An innovative strategy for immobilization of receptor proteins on to an optical fiber by use of poly(pyrrole-biotin)

Robert S. Marks, Andres Novoa, David Thomassey, Serge Cosnier

    Research output: Contribution to journalArticlepeer-review

    35 Scopus citations

    Abstract

    We report an innovative and useful procedure for immobilization of antibodies on to a fiber-optic silica surface. The procedure consists in the chemical oxidation of pyrrole-biotin monomers that are readily deposited as a thin film of poly(pyrrole-biotin) polymer on to the end-face of the fiber. The film was shown to be sufficiently translucent to enable photon coupling within the fiber transducer and its presence was demonstrated by means of fluorescent micrographs of bound rhodamine-labeled avidin. Fiber-optics modified with cholera toxin B subunit molecules were tested for sensitivity, non-specificity, and overall practicality. It was shown that the fiber-optic immunoassay for the detection of anti-cholera toxin antibody was up to three orders of magnitude more sensitive than the classical enzyme-linked immunosorbent assay (ELISA).

    Original languageEnglish
    Pages (from-to)1056-1063
    Number of pages8
    JournalAnalytical and Bioanalytical Chemistry
    Volume374
    Issue number6
    DOIs
    StatePublished - 24 Dec 2002

    Keywords

    • Anti-cholera toxin antibody
    • Biotinylated polypyrrole
    • Chemiluminescence
    • Diagnostics
    • Fiber optic immunosensors
    • Protein immobilization

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry

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