An optimized protocol for the retroviral transduction of mouse CD4 T cells

Ekaterina Eremenko, Zoe V. Taylor, Bishnu Khand, Shir Zaccai, Angel Porgador, Alon Monsonego

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death. For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.

Original languageEnglish
Article number100719
JournalSTAR Protocols
Volume2
Issue number3
DOIs
StatePublished - 17 Sep 2021

Keywords

  • Cell culture
  • Cell separation/fractionation
  • Cell-based Assays
  • Immunology
  • Molecular Biology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Neuroscience (all)
  • Immunology and Microbiology (all)
  • Medicine (all)

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