An unspliced group I intron in 23S rRNA links Chlamydiales, chloroplasts, and mitochondria

Karin D.E. Everett, Simona Kahane, Robin M. Bush, Maureen G. Friedman

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Chlamydia was the only genus in the order Chlamydiales until the recent characterization of Simkania negevensis Z(T) and Parachlamydia acanthamoebae strains. The present study of Chlamydiales 23S ribosomal DNA (rDNA) focuses on a naturally occurring group I intron in the I-CpaI target site of 23S rDNA from S. negevensis. The intron, SnLSU · 1, belonged to the IB4 structural subgroup and was most closely related to large ribosomal subunit introns that express single-motif, LAGLIDADG endonucleases in chloroplasts of algae and in mitochondria of amoebae. RT-PCR and electrophoresis of in vivo rRNA indicated that the intron was not spliced out of the 23S rRNA. The unspliced 658-nt intron is the first group I intron to be found in bacterial rDNA or rRNA, and it may delay the S. negevensis developmental replication cycle by affecting ribosomal function.

Original languageEnglish
Pages (from-to)4734-4740
Number of pages7
JournalJournal of Bacteriology
Volume181
Issue number16
DOIs
StatePublished - 1 Jan 1999

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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