Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy

B. H. Laster; E. A. Popenoe; L. Wielopolski; S. L. Commerford; R. Gahbauer; J. Goodman; A. Meek; R. G. Fairchild

doi:10.1016/0167-8140(90)90130-O

Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy

B. H. Laster, E. A. Popenoe, L. Wielopolski, S. L. Commerford, R. Gahbauer, J. Goodman, A. Meek, R. G. Fairchild

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion apparatus capable of delivering continuous multi-day infusions without restraining the mice. The absolute incorporation of IdUrd in DNA was measured by ¹²⁵IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and ¹²⁵I counting showed good agreement.

Original language	English
Pages (from-to)	169-178
Number of pages	10
Journal	Radiotherapy and Oncology
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0167-8140(90)90130-O
State	Published - 1 Jan 1990
Externally published	Yes

Keywords

Continuous infusion
IdUrd
Neutron activation analysis
Nucleoside analog substitution
Photon activation therapy

ASJC Scopus subject areas

Hematology
Oncology
Radiology Nuclear Medicine and imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0167-8140(90)90130-O

Cite this

@article{8a40b277196549c3ad171f86c4357df3,

title = "Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy",

abstract = "Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion apparatus capable of delivering continuous multi-day infusions without restraining the mice. The absolute incorporation of IdUrd in DNA was measured by 125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and 125I counting showed good agreement.",

keywords = "Continuous infusion, IdUrd, Neutron activation analysis, Nucleoside analog substitution, Photon activation therapy",

author = "Laster, {B. H.} and Popenoe, {E. A.} and L. Wielopolski and Commerford, {S. L.} and R. Gahbauer and J. Goodman and A. Meek and Fairchild, {R. G.}",

note = "Funding Information: The authors are deeply grateful to Chris Gordon and Lynne Warkentien for their outstanding technical assistance, to Dr. Kengo Matsui for his advice in developing the infusion technique, to Dr. Irwin Fand for preparing the 12{\textquoteright}IdUrd autoradiographs, and to Bernice Armstrong and Lori Ann Zein for their patience in preparing the manuscript. Research was carried out under the auspices of the U.S. Dept. of Energy Contract No. DE-AC02-76CH00016 and NIH Grant No. CA32016.",

year = "1990",

month = jan,

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doi = "10.1016/0167-8140(90)90130-O",

language = "English",

volume = "19",

pages = "169--178",

journal = "Radiotherapy and Oncology",

issn = "0167-8140",

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TY - JOUR

T1 - Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy

AU - Laster, B. H.

AU - Popenoe, E. A.

AU - Wielopolski, L.

AU - Commerford, S. L.

AU - Gahbauer, R.

AU - Goodman, J.

AU - Meek, A.

AU - Fairchild, R. G.

N1 - Funding Information: The authors are deeply grateful to Chris Gordon and Lynne Warkentien for their outstanding technical assistance, to Dr. Kengo Matsui for his advice in developing the infusion technique, to Dr. Irwin Fand for preparing the 12’IdUrd autoradiographs, and to Bernice Armstrong and Lori Ann Zein for their patience in preparing the manuscript. Research was carried out under the auspices of the U.S. Dept. of Energy Contract No. DE-AC02-76CH00016 and NIH Grant No. CA32016.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion apparatus capable of delivering continuous multi-day infusions without restraining the mice. The absolute incorporation of IdUrd in DNA was measured by 125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and 125I counting showed good agreement.

AB - Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion apparatus capable of delivering continuous multi-day infusions without restraining the mice. The absolute incorporation of IdUrd in DNA was measured by 125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and 125I counting showed good agreement.

KW - Continuous infusion

KW - IdUrd

KW - Neutron activation analysis

KW - Nucleoside analog substitution

KW - Photon activation therapy

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DO - 10.1016/0167-8140(90)90130-O

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C2 - 2255770

AN - SCOPUS:0025044047

SN - 0167-8140

VL - 19

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JF - Radiotherapy and Oncology

IS - 2

ER -

Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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