Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster, Ashley Elliott, Arik Dahan, Jonathan M. Miller

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

    Original languageEnglish
    Pages (from-to)742-744
    Number of pages3
    JournalAnalytical Methods
    Volume3
    Issue number3
    DOIs
    StatePublished - 1 Mar 2011

    ASJC Scopus subject areas

    • Analytical Chemistry
    • General Chemical Engineering
    • General Engineering

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