Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster; Ashley Elliott; Arik Dahan; Jonathan M. Miller

doi:10.1039/c0ay00698j

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster
, Ashley Elliott
, Arik Dahan
, Jonathan M. Miller

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

Original language	English
Pages (from-to)	742-744
Number of pages	3
Journal	Analytical Methods
Volume	3
Issue number	3
DOIs	https://doi.org/10.1039/c0ay00698j
State	Published - 1 Mar 2011

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
General Engineering

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10.1039/c0ay00698j

Cite this

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title = "Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection",

abstract = "A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent{\textregistered} Diamond Hydride column.",

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year = "2011",

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doi = "10.1039/c0ay00698j",

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T1 - Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

AU - Webster, Gregory K.

AU - Elliott, Ashley

AU - Dahan, Arik

AU - Miller, Jonathan M.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

AB - A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

UR - https://www.scopus.com/pages/publications/79952948327

U2 - 10.1039/c0ay00698j

DO - 10.1039/c0ay00698j

M3 - Article

AN - SCOPUS:79952948327

SN - 1759-9660

VL - 3

SP - 742

EP - 744

JO - Analytical Methods

JF - Analytical Methods

IS - 3

ER -

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Abstract

ASJC Scopus subject areas

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