Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method

Hao Chen; Chang Liu; Yan Li; Si Wu; Yi Feng Chai

doi:10.5428/pcar20110612

Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method

Hao Chen, Chang Liu, Yan Li, Si Wu, Yi Feng Chai

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Objective: To analyze the chemical constituents of Hedan tablet by high performance liquid chromatography-time of flight-mass spectrometry (HPLC-TOF-MS). Methods: The separation was performed on a Agilent Zorbax SB-Aq column (250 mmx4.6 mm, 5 μm). The mobile phase consisting of acetonitrile (A)-1% acetic acid water solution (B) was used as gradient elute, 0-50 min, 0%-30% B, 50-90 min, 30%-75% B. The flow rate was 1 ml/min. TOF-MS was applied for qualitative analysis in positive ion mode with m/z of reference fragment 121.05 and 922.01. Results: The 33 chemical constituents of Hedan tablet were identified, and 5 groups of isomers were discriminated by TOF-MS and structure-relevant fragment ions. Conclusion: The HPLC-TOF-MS method is simple and reliable for analysis of the chemical constituents of Hedan tablets.

Original language	English
Pages (from-to)	439-443
Number of pages	5
Journal	Pharmaceutical Care and Research
Volume	11
Issue number	6
DOIs	https://doi.org/10.5428/pcar20110612
State	Published - 1 Jan 2011
Externally published	Yes

Keywords

Chemical constituents
Chromatography, high performance liquid
Hedan tablet
Mass spectrometry, tandem

ASJC Scopus subject areas

Pharmacy
Pharmacology
Pharmaceutical Science
Drug Discovery

Access to Document

10.5428/pcar20110612

Cite this

@article{dcf721f07c62487fb355eba29c1ba6c3,

title = "Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method",

abstract = "Objective: To analyze the chemical constituents of Hedan tablet by high performance liquid chromatography-time of flight-mass spectrometry (HPLC-TOF-MS). Methods: The separation was performed on a Agilent Zorbax SB-Aq column (250 mmx4.6 mm, 5 μm). The mobile phase consisting of acetonitrile (A)-1% acetic acid water solution (B) was used as gradient elute, 0-50 min, 0%-30% B, 50-90 min, 30%-75% B. The flow rate was 1 ml/min. TOF-MS was applied for qualitative analysis in positive ion mode with m/z of reference fragment 121.05 and 922.01. Results: The 33 chemical constituents of Hedan tablet were identified, and 5 groups of isomers were discriminated by TOF-MS and structure-relevant fragment ions. Conclusion: The HPLC-TOF-MS method is simple and reliable for analysis of the chemical constituents of Hedan tablets.",

keywords = "Chemical constituents, Chromatography, high performance liquid, Hedan tablet, Mass spectrometry, tandem",

author = "Hao Chen and Chang Liu and Yan Li and Si Wu and Chai, {Yi Feng}",

year = "2011",

month = jan,

day = "1",

doi = "10.5428/pcar20110612",

language = "English",

volume = "11",

pages = "439--443",

journal = "Pharmaceutical Care and Research",

issn = "1671-2838",

publisher = "Publishing House of Pharmaceutical Care and Research",

number = "6",

}

TY - JOUR

T1 - Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method

AU - Chen, Hao

AU - Liu, Chang

AU - Li, Yan

AU - Wu, Si

AU - Chai, Yi Feng

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Objective: To analyze the chemical constituents of Hedan tablet by high performance liquid chromatography-time of flight-mass spectrometry (HPLC-TOF-MS). Methods: The separation was performed on a Agilent Zorbax SB-Aq column (250 mmx4.6 mm, 5 μm). The mobile phase consisting of acetonitrile (A)-1% acetic acid water solution (B) was used as gradient elute, 0-50 min, 0%-30% B, 50-90 min, 30%-75% B. The flow rate was 1 ml/min. TOF-MS was applied for qualitative analysis in positive ion mode with m/z of reference fragment 121.05 and 922.01. Results: The 33 chemical constituents of Hedan tablet were identified, and 5 groups of isomers were discriminated by TOF-MS and structure-relevant fragment ions. Conclusion: The HPLC-TOF-MS method is simple and reliable for analysis of the chemical constituents of Hedan tablets.

AB - Objective: To analyze the chemical constituents of Hedan tablet by high performance liquid chromatography-time of flight-mass spectrometry (HPLC-TOF-MS). Methods: The separation was performed on a Agilent Zorbax SB-Aq column (250 mmx4.6 mm, 5 μm). The mobile phase consisting of acetonitrile (A)-1% acetic acid water solution (B) was used as gradient elute, 0-50 min, 0%-30% B, 50-90 min, 30%-75% B. The flow rate was 1 ml/min. TOF-MS was applied for qualitative analysis in positive ion mode with m/z of reference fragment 121.05 and 922.01. Results: The 33 chemical constituents of Hedan tablet were identified, and 5 groups of isomers were discriminated by TOF-MS and structure-relevant fragment ions. Conclusion: The HPLC-TOF-MS method is simple and reliable for analysis of the chemical constituents of Hedan tablets.

KW - Chemical constituents

KW - Chromatography, high performance liquid

KW - Hedan tablet

KW - Mass spectrometry, tandem

UR - http://www.scopus.com/inward/record.url?scp=84862913048&partnerID=8YFLogxK

U2 - 10.5428/pcar20110612

DO - 10.5428/pcar20110612

M3 - Article

AN - SCOPUS:84862913048

SN - 1671-2838

VL - 11

SP - 439

EP - 443

JO - Pharmaceutical Care and Research

JF - Pharmaceutical Care and Research

IS - 6

ER -

Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this