Analysis of the mechanical properties of wild type and hyperstable mutants of the HIV-1 capsid

Ruben Ramalho, Sanela Rankovic, Jing Zhou, Christopher Aiken, Itay Rousso

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Background: The human immunodeficiency virus (HIV-1) capsid is a self-assembled protein shell that contains the viral genome. During the stages between viral entry into a host cell and nuclear import of the viral DNA, the capsid dissociates in a process known as uncoating, which leads to the release of the viral genetic material. Mutations that alter the stability of the capsid affect the uncoating rate and impair HIV-1 infectivity. Results: To gain further insight into the role of capsid stability during uncoating, we used atomic force spectroscopy to quantify the stiffness of the capsid. Empty in vitro assemblies of wild type (WT) and mutant recombinant HIV-1 capsid protein (CA) as well as isolated WT and mutant HIV-1 cores (i.e., filled capsids) were analyzed. We find that hyperstable CA mutant assemblies (A204C, A14C/E45C, E45A and E45A/R132T) are significantly stiffer than WT assemblies. However, the hardening effect of disulfide crosslinking (A204C and A14C/E45C) is lower than that of hydrophobic interactions (E45A and E45A/R132T). Conclusions: Our results demonstrate that mutations that increase the intrinsic stability of the HIV-1 capsid have an increased stiffness of their lattice.

Original languageEnglish
Article number17
JournalRetrovirology
Volume13
Issue number1
DOIs
StatePublished - 15 Mar 2016

Keywords

  • Atomic force microscopy
  • Capsid
  • HIV
  • Stiffness

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