TY - JOUR
T1 - Analysis of two Mexican Pectobacterium brasiliense strains reveals an inverted relationship between c-di-GMP levels with exopolysaccharide production and swarming motility
AU - Narváez-Barragán, Delia A.
AU - de Sandozequi, Andrés
AU - Rodríguez, Mabel
AU - Estrada, Karel
AU - Tovar-Herrera, Omar E.
AU - Martínez-Anaya, Claudia
N1 - Funding Information:
We are grateful to Dr. Oscar Mascorro from Universidad de Chapingo for his kind donation of Pectobacterium strains BF20 and BF45, Dr. I. Toth from The James Hutton Institute for the kind donation of P. atrosepticum SCRI 1043, Dr. S.R. Long and Dr. M. Barnett from Stanford University for kindly providing plasmid PTB93 F, Dr. Miguel A. Ceballos and Dr. David Zamorano from CCG-UNAM for providing plasmids pZLR4 and pFY4535, respectively. To María Ines Isidro-Coxca and Dr. José Luis Puente from IBT-UNAM for providing plasmid pMPMT3-pdeH-3xFLAG. To Drs. David Zamorano, Lorenzo Segovia and Chris Wood for critical reading of this manuscript. Genome sequencing was performed by Ricardo Grande at Unidad Universitaria de Secuenciación Masiva y Bioinformática (IBT, UNAM). To Arturo Pimentel and Andrés Saralegui from Laboratorio Nacional de Microscopia Avanzada (LMNA, IBT-UNAM), and to Blanca Ramos Cerrillo and Ivana Blancas-Nava for technical assistance. D.N.-B., A.DS., M.R. and O.E.T.-H. received financial support from CONACYT, México fellowships. This work was financed by CONACYT Ciencia Básica, México ( 166050 and 252551 ) and PAPIIT-DGAPA , México (IN211116 and IN211019) grants to C.M.-A.
Funding Information:
We are grateful to Dr. Oscar Mascorro from Universidad de Chapingo for his kind donation of Pectobacterium strains BF20 and BF45, Dr. I. Toth from The James Hutton Institute for the kind donation of P. atrosepticum SCRI 1043, Dr. S.R. Long and Dr. M. Barnett from Stanford University for kindly providing plasmid PTB93 F, Dr. Miguel A. Ceballos and Dr. David Zamorano from CCG-UNAM for providing plasmids pZLR4 and pFY4535, respectively. To Mar?a Ines Isidro-Coxca and Dr. Jos? Luis Puente from IBT-UNAM for providing plasmid pMPMT3-pdeH-3xFLAG. To Drs. David Zamorano, Lorenzo Segovia and Chris Wood for critical reading of this manuscript. Genome sequencing was performed by Ricardo Grande at Unidad Universitaria de Secuenciaci?n Masiva y Bioinform?tica (IBT, UNAM). To Arturo Pimentel and Andr?s Saralegui from Laboratorio Nacional de Microscopia Avanzada (LMNA, IBT-UNAM), and to Blanca Ramos Cerrillo and Ivana Blancas-Nava for technical assistance. D.N.-B. A.DS. M.R. and O.E.T.-H. received financial support from CONACYT, M?xico fellowships. This work was financed by CONACYT Ciencia B?sica, M?xico (166050 and 252551) and PAPIIT-DGAPA, M?xico (IN211116 and IN211019) grants to C.M.-A.
Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Pectobacterium is a diverse genus of phytopathogenic species from soil and water that cause infection either to restricted or multiple plant hosts. Phylogenetic analysis and metabolic fingerprinting of large numbers of genomes have expanded classification of Pectobacterium members. Pectobacterium brasiliense sp. nov has been elevated to the species level having detached from P. carotovorum. Here we present two P. brasiliense strains BF20 and BF45 isolated in Mexico from Opuntia and tobacco, respectively, which cluster into two different groups in whole genome comparisons with other Pectobacterium. We found that BF20 and BF45 strains are phenotypically different as BF45 showed more severe and rapid symptoms in comparison to BF20 in the host models celery and broccoli. Both strains produced similar levels of the main autoinducers, but BF45 shows an additional low abundant autoinducer compared to strain BF20. The two strains had different levels of c-di-GMP, which regulates the transition from motile to sessile lifestyle. In contrast to BF45, BF20 had the highest levels of c-di-GMP, was more motile (swarming), non-flocculant and less proficient in biofilm formation and exopolysaccharide production. Genomic comparisons revealed that differences in c-di-GMP accumulation and perhaps the associated phenotypes might be due to unique c-di-GMP metabolic genes in these two strains. Our results improve our understanding of the associations between phenotype and genotype and how this has shaped the physiology of Pectobacterium strains.
AB - Pectobacterium is a diverse genus of phytopathogenic species from soil and water that cause infection either to restricted or multiple plant hosts. Phylogenetic analysis and metabolic fingerprinting of large numbers of genomes have expanded classification of Pectobacterium members. Pectobacterium brasiliense sp. nov has been elevated to the species level having detached from P. carotovorum. Here we present two P. brasiliense strains BF20 and BF45 isolated in Mexico from Opuntia and tobacco, respectively, which cluster into two different groups in whole genome comparisons with other Pectobacterium. We found that BF20 and BF45 strains are phenotypically different as BF45 showed more severe and rapid symptoms in comparison to BF20 in the host models celery and broccoli. Both strains produced similar levels of the main autoinducers, but BF45 shows an additional low abundant autoinducer compared to strain BF20. The two strains had different levels of c-di-GMP, which regulates the transition from motile to sessile lifestyle. In contrast to BF45, BF20 had the highest levels of c-di-GMP, was more motile (swarming), non-flocculant and less proficient in biofilm formation and exopolysaccharide production. Genomic comparisons revealed that differences in c-di-GMP accumulation and perhaps the associated phenotypes might be due to unique c-di-GMP metabolic genes in these two strains. Our results improve our understanding of the associations between phenotype and genotype and how this has shaped the physiology of Pectobacterium strains.
KW - Autoinducer
KW - C-Di-GMP
KW - Pectobacterium
KW - Swarming
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=85079860975&partnerID=8YFLogxK
U2 - 10.1016/j.micres.2020.126427
DO - 10.1016/j.micres.2020.126427
M3 - Article
C2 - 32109688
AN - SCOPUS:85079860975
VL - 235
JO - Microbiological Research
JF - Microbiological Research
SN - 0944-5013
M1 - 126427
ER -