TY - JOUR
T1 - Angiogenesis PET tracer uptake (68Ga-NODAGA-E[(cRGDyK)]2) in induced myocardial infarction and stromal cell treatment inminipigs
AU - Rasmussen, Thomas
AU - Follin, Bjarke
AU - Kastrup, Jens
AU - Brandt-Larsen, Malene
AU - Madsen, Jacob
AU - Christensen, Thomas Emil
AU - Juhl, Morten
AU - Cohen, Smadar
AU - Hammelev, Karsten Pharao
AU - Møller, Christian Holdflod
AU - Goetze, Jens Peter
AU - Hasbak, Philip
AU - Kjær, Andreas
N1 - Publisher Copyright:
© 2018 by the authors.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Angiogenesis is considered integral to the reparative process after ischemic injury. The αvβ3 integrin is a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68Ga-NODAGA-E[(cRGDyK)]2 (RGD) is a positron-emission-tomography (PET) ligand targeted towards αvβ3 integrin. The aim was to present data for the uptake of RGD and correlate it with histology and to further illustrate the differences in angiogenesis due to porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment in minipigs after induction of myocardial infarction (MI). Three minipigs were treated with direct intra-myocardial injection of pASCs and two minipigs with saline. MI was confirmed by 82Rubidium (82Rb) dipyridamole stress PET. Mean Standardized Uptake Values (SUVmean) of RGD were higher in the infarct compared to non-infarct area one week and one month after MI in both pASC-treated (SUVmean: 1.23 vs. 0.88 and 1.02 vs. 0.86, p < 0.05 for both) and non-pASC-treated minipigs (SUVmean: 1.44 vs. 1.07 and 1.26 vs. 1.04, p < 0.05 for both). However, there was no difference in RGD uptake, ejection fractions, coronary flow reserves or capillary density in histology between the two groups. In summary, indications of angiogenesis were present in the infarcted myocardium. However, no differences between pASC-treated and non-pASC-treated minipigs could be demonstrated.
AB - Angiogenesis is considered integral to the reparative process after ischemic injury. The αvβ3 integrin is a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68Ga-NODAGA-E[(cRGDyK)]2 (RGD) is a positron-emission-tomography (PET) ligand targeted towards αvβ3 integrin. The aim was to present data for the uptake of RGD and correlate it with histology and to further illustrate the differences in angiogenesis due to porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment in minipigs after induction of myocardial infarction (MI). Three minipigs were treated with direct intra-myocardial injection of pASCs and two minipigs with saline. MI was confirmed by 82Rubidium (82Rb) dipyridamole stress PET. Mean Standardized Uptake Values (SUVmean) of RGD were higher in the infarct compared to non-infarct area one week and one month after MI in both pASC-treated (SUVmean: 1.23 vs. 0.88 and 1.02 vs. 0.86, p < 0.05 for both) and non-pASC-treated minipigs (SUVmean: 1.44 vs. 1.07 and 1.26 vs. 1.04, p < 0.05 for both). However, there was no difference in RGD uptake, ejection fractions, coronary flow reserves or capillary density in histology between the two groups. In summary, indications of angiogenesis were present in the infarcted myocardium. However, no differences between pASC-treated and non-pASC-treated minipigs could be demonstrated.
KW - Angiogenesis
KW - Cardiac positron-emission-tomography
KW - Hydrogel
KW - Mesenchymal stromal cells
KW - Myocardial perfusion
KW - RGD
KW - Rubidium
KW - Stemcell;myocardial infarction
UR - http://www.scopus.com/inward/record.url?scp=85082510273&partnerID=8YFLogxK
U2 - 10.3390/diagnostics8020033
DO - 10.3390/diagnostics8020033
M3 - Article
AN - SCOPUS:85082510273
SN - 2075-4418
VL - 8
JO - Diagnostics
JF - Diagnostics
IS - 2
M1 - 33
ER -