TY - JOUR
T1 - Anthraquinones production in Rubia tinctorum cell suspension cultures
T2 - Down scale of shear effects
AU - Busto, Víctor D.
AU - Calabró-López, Ariel
AU - Rodríguez-Talou, Julián
AU - Giulietti, Ana M.
AU - Merchuk, José C.
N1 - Funding Information:
This work was financially supported by grant PICT 2005 33166 , Agencia Nacional de Promoción Científica y Tecnológica, Argentina (ANPCyT) , and by grant UBACyT B111 , Universidad de Buenos Aires. R.T.J., G.A.M. and B.D.V. belong to CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina). C.L.A. is a fellow from ANPCyT. The authors wish to thank Dr Sebastián Cavalitto (CINDEFI, Facultad de Ciencias Exactas, Universidad Nacional de la Plata) and Lic. Florencia Leal Denis (Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires) for technical support on k L a measurements.
PY - 2013/7/5
Y1 - 2013/7/5
N2 - The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) [39] was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360. rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450. rpm, previously published (Busto et al., 2008 [17]). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.
AB - The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) [39] was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360. rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450. rpm, previously published (Busto et al., 2008 [17]). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.
KW - Anthraquinones
KW - Plant cell cultures
KW - Rubia tinctorum
KW - Scale-down
KW - Shaken bioreactors
KW - Shear stress
KW - Turbulence
UR - http://www.scopus.com/inward/record.url?scp=84879570293&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2013.05.013
DO - 10.1016/j.bej.2013.05.013
M3 - Article
AN - SCOPUS:84879570293
SN - 1369-703X
VL - 77
SP - 119
EP - 128
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
ER -