Anti-osteoarthritic activity of Bungarus fasciatus venom fraction BF-F47 involving molecular markers in the rats

Antony Gomes; Susmita Ghosh; Sourav Ghosh; Kalyani Saha; Partha Pratim Saha; Subir Chandra Dasgupta; Aparna Gomes

doi:10.1016/j.toxicon.2016.04.039

Anti-osteoarthritic activity of Bungarus fasciatus venom fraction BF-F47 involving molecular markers in the rats

Antony Gomes, Susmita Ghosh, Sourav Ghosh, Kalyani Saha, Partha Pratim Saha, Subir Chandra Dasgupta, Aparna Gomes

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A heat stable protein BF-F47 was purified from the crude venom of Bungarus fasciatus by CM cellulose ion exchange chromatography and HPLC. Osteoarthritis (OA) was developed in male albino Wistar rats by collagenase injection. BF-F47 treatment significantly restored urinary hydroxyproline and glucosamine in OA rats. Serum acid phosphatase, alkaline phosphatase, creatinine and serum molecular markers TNF-α, IL-1β, IL-17, cytokine induced neutrophil chemoattractant-1, matrix metalloproteinase-1, cathepsin-K, osteocalcin and PGE2 were also significantly altered. BF-F47 showed partial restoration of osteoarthritis joints. Thus, BF-F47 induced anti-osteoarthritic activity in Wistar rats acted through molecular markers of arthritis and inflammation.

Original language	English
Pages (from-to)	43-46
Number of pages	4
Journal	Toxicon
Volume	118
DOIs	https://doi.org/10.1016/j.toxicon.2016.04.039
State	Published - 1 Aug 2016
Externally published	Yes

Keywords

Arthritis
Banded krait
Bungarus fasciatus
Inflammation
Osteoarthritis
Snake venom

ASJC Scopus subject areas

Toxicology

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10.1016/j.toxicon.2016.04.039

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title = "Anti-osteoarthritic activity of Bungarus fasciatus venom fraction BF-F47 involving molecular markers in the rats",

abstract = "A heat stable protein BF-F47 was purified from the crude venom of Bungarus fasciatus by CM cellulose ion exchange chromatography and HPLC. Osteoarthritis (OA) was developed in male albino Wistar rats by collagenase injection. BF-F47 treatment significantly restored urinary hydroxyproline and glucosamine in OA rats. Serum acid phosphatase, alkaline phosphatase, creatinine and serum molecular markers TNF-α, IL-1β, IL-17, cytokine induced neutrophil chemoattractant-1, matrix metalloproteinase-1, cathepsin-K, osteocalcin and PGE2 were also significantly altered. BF-F47 showed partial restoration of osteoarthritis joints. Thus, BF-F47 induced anti-osteoarthritic activity in Wistar rats acted through molecular markers of arthritis and inflammation.",

keywords = "Arthritis, Banded krait, Bungarus fasciatus, Inflammation, Osteoarthritis, Snake venom",

author = "Antony Gomes and Susmita Ghosh and Sourav Ghosh and Kalyani Saha and Saha, {Partha Pratim} and Dasgupta, {Subir Chandra} and Aparna Gomes",

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doi = "10.1016/j.toxicon.2016.04.039",

language = "English",

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AU - Gomes, Antony

AU - Ghosh, Susmita

AU - Ghosh, Sourav

AU - Saha, Kalyani

AU - Saha, Partha Pratim

AU - Dasgupta, Subir Chandra

AU - Gomes, Aparna

PY - 2016/8/1

Y1 - 2016/8/1

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AB - A heat stable protein BF-F47 was purified from the crude venom of Bungarus fasciatus by CM cellulose ion exchange chromatography and HPLC. Osteoarthritis (OA) was developed in male albino Wistar rats by collagenase injection. BF-F47 treatment significantly restored urinary hydroxyproline and glucosamine in OA rats. Serum acid phosphatase, alkaline phosphatase, creatinine and serum molecular markers TNF-α, IL-1β, IL-17, cytokine induced neutrophil chemoattractant-1, matrix metalloproteinase-1, cathepsin-K, osteocalcin and PGE2 were also significantly altered. BF-F47 showed partial restoration of osteoarthritis joints. Thus, BF-F47 induced anti-osteoarthritic activity in Wistar rats acted through molecular markers of arthritis and inflammation.

KW - Arthritis

KW - Banded krait

KW - Bungarus fasciatus

KW - Inflammation

KW - Osteoarthritis

KW - Snake venom

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SN - 0041-0101

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JO - Toxicon

JF - Toxicon

ER -

Anti-osteoarthritic activity of Bungarus fasciatus venom fraction BF-F47 involving molecular markers in the rats

Abstract

Keywords

ASJC Scopus subject areas

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