TY - JOUR
T1 - Antibiofilm activity of 3,3'-diindolylmethane on Staphylococcus aureus and its disinfection on common food-contact surfaces
AU - Zhang, Hui
AU - Guo, Xiaomei
AU - Tian, Lei
AU - Wang, Na
AU - Li, Yuqing
AU - Kushmaro, Ariel
AU - Marks, Robert
AU - Sun, Qun
N1 - Funding Information:
This work was supported by the National Key Research and Development Projects (2019YFE0103800); Sichuan Science and Technology Program (2019YFH0113, 2021YFH0060, 2021YFH0072, 2021ZHFP0045, 2021YFN0092); and the Fundamental Research Funds for the Central Universities (2018CDLZ-07, 2018CDPZH-9, 2019CDPZH-23, 2020CDLZ-17).
Publisher Copyright:
© 2022
PY - 2022/9/1
Y1 - 2022/9/1
N2 - This study explored the antibiofilm efficacy of 3,3'-diindolylmethane (DIM) on Staphylococcus aureus and its disinfection on common food-contact surfaces. The minimum biofilm inhibitory concentration (MBIC) of DIM on S. aureus was 62.5 μmol/L, while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay. DIM in the concentration range of 31.2−62.5 μmol/L demonstrated a dose-dependent antibiofilm activity to S. aureus, as confirmed by light microscopic (LM), confocal laser scanning microscopic (CLSM), and scanning electron microscopic (SEM) analyses. At DIM of 62.5 μmol/L, the biomass of S. aureus biofilm was significantly reduced by 97 % and its average thickness by 58 % (P < 0.05). DIM of 62.5 μmol/L inhibited the bacterial initial adhesion and proliferation, as well as cell motility; the release of extracellular DNA (eDNA) and extracellular polysaccharide (EPS) were reduced by 75 % and 69 %, respectively. DIM exhibited a strong inhibition to S. aureus biofilm formation on common food-contact surfaces, including 304 stainless steel, glass, and polyvinyl chloride (PVC) but not disperse the mature biofilm. Overall, our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S. aureus on common food-contact surfaces utilized during food processing.
AB - This study explored the antibiofilm efficacy of 3,3'-diindolylmethane (DIM) on Staphylococcus aureus and its disinfection on common food-contact surfaces. The minimum biofilm inhibitory concentration (MBIC) of DIM on S. aureus was 62.5 μmol/L, while it did not impede the bacterial growth evaluated by growth curve and XTT reduction assay. DIM in the concentration range of 31.2−62.5 μmol/L demonstrated a dose-dependent antibiofilm activity to S. aureus, as confirmed by light microscopic (LM), confocal laser scanning microscopic (CLSM), and scanning electron microscopic (SEM) analyses. At DIM of 62.5 μmol/L, the biomass of S. aureus biofilm was significantly reduced by 97 % and its average thickness by 58 % (P < 0.05). DIM of 62.5 μmol/L inhibited the bacterial initial adhesion and proliferation, as well as cell motility; the release of extracellular DNA (eDNA) and extracellular polysaccharide (EPS) were reduced by 75 % and 69 %, respectively. DIM exhibited a strong inhibition to S. aureus biofilm formation on common food-contact surfaces, including 304 stainless steel, glass, and polyvinyl chloride (PVC) but not disperse the mature biofilm. Overall, our investigation identified DIM as a promising antibiofilm agent and its suitability to prevent the biofilm formation of S. aureus on common food-contact surfaces utilized during food processing.
KW - 3,3'-Diindolylmethane
KW - Biofilm inhibition
KW - Extracellular DNA
KW - Extracellular polysaccharide
KW - Food-contact surfaces
UR - http://www.scopus.com/inward/record.url?scp=85132403433&partnerID=8YFLogxK
U2 - 10.1016/j.fshw.2022.04.017
DO - 10.1016/j.fshw.2022.04.017
M3 - Article
AN - SCOPUS:85132403433
SN - 2213-4530
VL - 11
SP - 1222
EP - 1232
JO - Food Science and Human Wellness
JF - Food Science and Human Wellness
IS - 5
ER -