Antibody-based immobilization of bioluminescent bacterial sensor cells

J. Rajan Premkumar, Ovadia Lev, Robert S. Marks, Boris Polyak, Rachel Rosen, Shimshon Belkin

    Research output: Contribution to journalArticlepeer-review

    58 Scopus citations

    Abstract

    Whole-cell luminescent bioreporter sensors based on immobilized recombinant Escherichia coli are described and evaluated. The sensors were prepared by glutaraldehyde-anchoring of nonspecific anti-E. coli antibodies on aminosylilated gold or silica glass surfaces with subsequent attachment of the probe bacteria. We demonstrate the generality of the concept by attachment of several E. coli strains that express luciferase in response to different physiological stress conditions including heat shock, DNA damage (SOS), fatty acid availability, peroxide and oxidative stress. The sensors can be used either as single- or multiple-use disposable sensing elements or for continuous operation. We show compatibility with optical fiber technology. Storage stability of the sensors exceeded 5 months with no measurable deterioration of the signal. Repeatability on exposure in successive days was < 15%, as was sensor to sensor reproducibility. Sensitivity and detection limits of the immobilized cells were comparable to that of non-immobilized bacteria.

    Original languageEnglish
    Pages (from-to)1029-1038
    Number of pages10
    JournalTalanta
    Volume55
    Issue number5
    DOIs
    StatePublished - 13 Dec 2001

    Keywords

    • Antibody
    • Bioluminescence
    • E. coli
    • Sensor

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