Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria

Sandra De Lamo Marin; Yang Xu; Michael M. Meijler; Kim D. Janda

doi:10.1016/j.bmcl.2006.12.118

Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria

Sandra De Lamo Marin, Yang Xu, Michael M. Meijler, Kim D. Janda

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest k_cat, with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.

Original language	English
Pages (from-to)	1549-1552
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/j.bmcl.2006.12.118
State	Published - 15 Mar 2007
Externally published	Yes

Keywords

Catalytic antibodies
Homoserine lactone hydrolysis
Inhibitor
Quorum sensing

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2006.12.118

Cite this

@article{f86abd9ea499436194e3564f2e7f3d8e,

title = "Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria",

abstract = "N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest kcat, with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.",

keywords = "Catalytic antibodies, Homoserine lactone hydrolysis, Inhibitor, Quorum sensing",

author = "{De Lamo Marin}, Sandra and Yang Xu and Meijler, {Michael M.} and Janda, {Kim D.}",

note = "Funding Information: We thank Dr. Barbara H. Iglewski of the University of Rochester for the generous gift of wild-type P. aeruginosa strain PAO1. This work was supported by the National Institutes of Health (AI055781) and The Skaggs Institute for Chemical Biology.",

year = "2007",

month = mar,

day = "15",

doi = "10.1016/j.bmcl.2006.12.118",

language = "English",

volume = "17",

pages = "1549--1552",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd.",

number = "6",

}

TY - JOUR

T1 - Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria

AU - De Lamo Marin, Sandra

AU - Xu, Yang

AU - Meijler, Michael M.

AU - Janda, Kim D.

N1 - Funding Information: We thank Dr. Barbara H. Iglewski of the University of Rochester for the generous gift of wild-type P. aeruginosa strain PAO1. This work was supported by the National Institutes of Health (AI055781) and The Skaggs Institute for Chemical Biology.

PY - 2007/3/15

Y1 - 2007/3/15

N2 - N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest kcat, with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.

AB - N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest kcat, with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.

KW - Catalytic antibodies

KW - Homoserine lactone hydrolysis

KW - Inhibitor

KW - Quorum sensing

UR - http://www.scopus.com/inward/record.url?scp=33847079152&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2006.12.118

DO - 10.1016/j.bmcl.2006.12.118

M3 - Article

AN - SCOPUS:33847079152

SN - 0960-894X

VL - 17

SP - 1549

EP - 1552

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 6

ER -

Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this