Antibody-Functionalized Nanowires: A Tuner for the Activation of T Cells

Viraj Bhingardive, Anna Kossover, Muhammed Iraqi, Bishnu Khand, Guillaume Le Saux, Angel Porgador, Mark Schvartzman

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

T cells sense both chemical cues delivered by antigen molecules and physical cues delivered by the environmental elasticity and topography; yet, it is still largely unclear how these cues cumulatively regulate the immune activity of T cells. Here, we engineered a nanoscale platform for ex vivo stimulation of T cells based on antigen-functionalized nanowires. The nanowire topography and elasticity, as well as the immobilized antigens, deliver the physical and chemical cues, respectively, enabling the systematic study of the integrated effect of these cues on a T cell's immune response. We found that T cells sense both the topography and bending modulus of the nanowires and modulate their signaling, degranulation, and cytotoxicity with the variation in these physical features. Our study provides an important insight into the physical mechanism of T cell activation and paves the way to novel nanomaterials for the controlled ex vivo activation of T cells in immunotherapy.

Original languageEnglish
Pages (from-to)4241-4248
Number of pages8
JournalNano Letters
Volume21
Issue number10
DOIs
StatePublished - 26 May 2021

Keywords

  • T cells
  • biofunctionalization
  • immunotherapy
  • mechanosensing
  • nanowires

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