Antibody interference with N-Acyl homoserine lactone-mediated bacterial quorum sensing

Gunnar F. Kaufmann; Rafaella Sartorio; Sang Hyeup Lee; Jenny M. Mee; Laurence J. Altobell; David P. Kujawa; Emily Jeffries; Bruce Clapham; Michael M. Meijler; Kim D. Janda

doi:10.1021/ja0578698

Antibody interference with N-Acyl homoserine lactone-mediated bacterial quorum sensing

Gunnar F. Kaufmann, Rafaella Sartorio, Sang Hyeup Lee, Jenny M. Mee, Laurence J. Altobell, David P. Kujawa, Emily Jeffries, Bruce Clapham, Michael M. Meijler, Kim D. Janda

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

Many bacterial pathogens coordinate their virulence factor expression in a cell density-dependent manner. This population-dependent coordination of gene expression in bacteria has been termed "quorum sensing" (QS). N-Acyl homoserine lactones (AHLs) are used by over 70 Gram-negative bacterial species as autoinducers. Inhibition of QS signaling might represent a new target for antimicrobial therapy. Here we report the hapten design, synthesis, generation of monoclonal antibodies (mAbs) against AHLs, and the evaluation of these mAbs for their ability to blunt QS signaling and inhibit virulence factor expression in P. aeruginosa. The mAbs can be envisioned as a tool for future investigations into AHL-based QS, which may aid in gaining new insights into the pathogenesis of P. aeruginosa and may ultimately lead to the development of new strategies to combat bacterial diseases.

Original language	English
Pages (from-to)	2802-2803
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	9
DOIs	https://doi.org/10.1021/ja0578698
State	Published - 8 Mar 2006
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja0578698

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title = "Antibody interference with N-Acyl homoserine lactone-mediated bacterial quorum sensing",

abstract = "Many bacterial pathogens coordinate their virulence factor expression in a cell density-dependent manner. This population-dependent coordination of gene expression in bacteria has been termed {"}quorum sensing{"} (QS). N-Acyl homoserine lactones (AHLs) are used by over 70 Gram-negative bacterial species as autoinducers. Inhibition of QS signaling might represent a new target for antimicrobial therapy. Here we report the hapten design, synthesis, generation of monoclonal antibodies (mAbs) against AHLs, and the evaluation of these mAbs for their ability to blunt QS signaling and inhibit virulence factor expression in P. aeruginosa. The mAbs can be envisioned as a tool for future investigations into AHL-based QS, which may aid in gaining new insights into the pathogenesis of P. aeruginosa and may ultimately lead to the development of new strategies to combat bacterial diseases.",

author = "Kaufmann, {Gunnar F.} and Rafaella Sartorio and Lee, {Sang Hyeup} and Mee, {Jenny M.} and Altobell, {Laurence J.} and Kujawa, {David P.} and Emily Jeffries and Bruce Clapham and Meijler, {Michael M.} and Janda, {Kim D.}",

year = "2006",

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doi = "10.1021/ja0578698",

language = "English",

volume = "128",

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TY - JOUR

T1 - Antibody interference with N-Acyl homoserine lactone-mediated bacterial quorum sensing

AU - Kaufmann, Gunnar F.

AU - Sartorio, Rafaella

AU - Lee, Sang Hyeup

AU - Mee, Jenny M.

AU - Altobell, Laurence J.

AU - Kujawa, David P.

AU - Jeffries, Emily

AU - Clapham, Bruce

AU - Meijler, Michael M.

AU - Janda, Kim D.

PY - 2006/3/8

Y1 - 2006/3/8

N2 - Many bacterial pathogens coordinate their virulence factor expression in a cell density-dependent manner. This population-dependent coordination of gene expression in bacteria has been termed "quorum sensing" (QS). N-Acyl homoserine lactones (AHLs) are used by over 70 Gram-negative bacterial species as autoinducers. Inhibition of QS signaling might represent a new target for antimicrobial therapy. Here we report the hapten design, synthesis, generation of monoclonal antibodies (mAbs) against AHLs, and the evaluation of these mAbs for their ability to blunt QS signaling and inhibit virulence factor expression in P. aeruginosa. The mAbs can be envisioned as a tool for future investigations into AHL-based QS, which may aid in gaining new insights into the pathogenesis of P. aeruginosa and may ultimately lead to the development of new strategies to combat bacterial diseases.

AB - Many bacterial pathogens coordinate their virulence factor expression in a cell density-dependent manner. This population-dependent coordination of gene expression in bacteria has been termed "quorum sensing" (QS). N-Acyl homoserine lactones (AHLs) are used by over 70 Gram-negative bacterial species as autoinducers. Inhibition of QS signaling might represent a new target for antimicrobial therapy. Here we report the hapten design, synthesis, generation of monoclonal antibodies (mAbs) against AHLs, and the evaluation of these mAbs for their ability to blunt QS signaling and inhibit virulence factor expression in P. aeruginosa. The mAbs can be envisioned as a tool for future investigations into AHL-based QS, which may aid in gaining new insights into the pathogenesis of P. aeruginosa and may ultimately lead to the development of new strategies to combat bacterial diseases.

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DO - 10.1021/ja0578698

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 9

ER -

Antibody interference with N-Acyl homoserine lactone-mediated bacterial quorum sensing

Abstract

ASJC Scopus subject areas

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