Antimicrobial evaluation of polytetrafluoroethylene used as part of temporary restorations: An ex vivo study

Michael Solomonov; Dan Henry Levy; Alin Yaya; Joe Ben Itzhak; David Polak

doi:10.1111/aej.12617

Antimicrobial evaluation of polytetrafluoroethylene used as part of temporary restorations: An ex vivo study

Michael Solomonov
, Dan Henry Levy
, Alin Yaya
, Joe Ben Itzhak
, David Polak

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The use of polytetrafluoroethylene (PTFE) tape as the base layer of temporary restorations had gained popularity mainly due to the ease of manipulation. The aim of this study was to assess whether this method changes the potential for bacterial growth and leakage of temporary restorations. The direct contact test and live/dead fluorescent staining were used for comparing Enterococcus faecalis growth and biofilm formation on PTFE, composite, intermediate restorative material (IRM) and Coltosol F. Confocal laser scanning microscopy was employed to evaluate E. faecalis penetration through 2 mm of PTFE, IRM or Coltosol F placed on the bottom of the pulp chamber and into radicular dentinal tubules in extracted maxillary third molars. The results demonstrated that E. faecalis grows on and penetrates through PTFE significantly more than it does with IRM and Coltosol F, revealing its comparably reduced overall antimicrobial sealing ability when placed as the base part of temporary restorations.

Original language	English
Pages (from-to)	98-104
Number of pages	7
Journal	Australian Endodontic Journal
Volume	48
Issue number	1
DOIs	https://doi.org/10.1111/aej.12617
State	Published - 1 Apr 2022
Externally published	Yes

Keywords

bacterial penetration
biofilm
polytetrafluoroethylene
temporary restoration

ASJC Scopus subject areas

General Dentistry

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10.1111/aej.12617

Cite this

@article{311f5d97dc9046dbb657f6311b85ea32,

title = "Antimicrobial evaluation of polytetrafluoroethylene used as part of temporary restorations: An ex vivo study",

abstract = "The use of polytetrafluoroethylene (PTFE) tape as the base layer of temporary restorations had gained popularity mainly due to the ease of manipulation. The aim of this study was to assess whether this method changes the potential for bacterial growth and leakage of temporary restorations. The direct contact test and live/dead fluorescent staining were used for comparing Enterococcus faecalis growth and biofilm formation on PTFE, composite, intermediate restorative material (IRM) and Coltosol F. Confocal laser scanning microscopy was employed to evaluate E. faecalis penetration through 2 mm of PTFE, IRM or Coltosol F placed on the bottom of the pulp chamber and into radicular dentinal tubules in extracted maxillary third molars. The results demonstrated that E. faecalis grows on and penetrates through PTFE significantly more than it does with IRM and Coltosol F, revealing its comparably reduced overall antimicrobial sealing ability when placed as the base part of temporary restorations.",

keywords = "bacterial penetration, biofilm, polytetrafluoroethylene, temporary restoration",

author = "Michael Solomonov and Levy, \{Dan Henry\} and Alin Yaya and \{Ben Itzhak\}, Joe and David Polak",

note = "Publisher Copyright: {\textcopyright} 2022 Australian Society of Endodontology Inc.",

year = "2022",

month = apr,

day = "1",

doi = "10.1111/aej.12617",

language = "English",

volume = "48",

pages = "98--104",

journal = "Australian Endodontic Journal",

issn = "1329-1947",

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}

TY - JOUR

T1 - Antimicrobial evaluation of polytetrafluoroethylene used as part of temporary restorations

T2 - An ex vivo study

AU - Solomonov, Michael

AU - Levy, Dan Henry

AU - Yaya, Alin

AU - Ben Itzhak, Joe

AU - Polak, David

PY - 2022/4/1

Y1 - 2022/4/1

N2 - The use of polytetrafluoroethylene (PTFE) tape as the base layer of temporary restorations had gained popularity mainly due to the ease of manipulation. The aim of this study was to assess whether this method changes the potential for bacterial growth and leakage of temporary restorations. The direct contact test and live/dead fluorescent staining were used for comparing Enterococcus faecalis growth and biofilm formation on PTFE, composite, intermediate restorative material (IRM) and Coltosol F. Confocal laser scanning microscopy was employed to evaluate E. faecalis penetration through 2 mm of PTFE, IRM or Coltosol F placed on the bottom of the pulp chamber and into radicular dentinal tubules in extracted maxillary third molars. The results demonstrated that E. faecalis grows on and penetrates through PTFE significantly more than it does with IRM and Coltosol F, revealing its comparably reduced overall antimicrobial sealing ability when placed as the base part of temporary restorations.

AB - The use of polytetrafluoroethylene (PTFE) tape as the base layer of temporary restorations had gained popularity mainly due to the ease of manipulation. The aim of this study was to assess whether this method changes the potential for bacterial growth and leakage of temporary restorations. The direct contact test and live/dead fluorescent staining were used for comparing Enterococcus faecalis growth and biofilm formation on PTFE, composite, intermediate restorative material (IRM) and Coltosol F. Confocal laser scanning microscopy was employed to evaluate E. faecalis penetration through 2 mm of PTFE, IRM or Coltosol F placed on the bottom of the pulp chamber and into radicular dentinal tubules in extracted maxillary third molars. The results demonstrated that E. faecalis grows on and penetrates through PTFE significantly more than it does with IRM and Coltosol F, revealing its comparably reduced overall antimicrobial sealing ability when placed as the base part of temporary restorations.

KW - bacterial penetration

KW - biofilm

KW - polytetrafluoroethylene

KW - temporary restoration

UR - https://www.scopus.com/pages/publications/85126042599

U2 - 10.1111/aej.12617

DO - 10.1111/aej.12617

M3 - Article

C2 - 35267229

AN - SCOPUS:85126042599

SN - 1329-1947

VL - 48

SP - 98

EP - 104

JO - Australian Endodontic Journal

JF - Australian Endodontic Journal

IS - 1

ER -

Antimicrobial evaluation of polytetrafluoroethylene used as part of temporary restorations: An ex vivo study

Abstract

Keywords

ASJC Scopus subject areas

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