Antitumor Activity of Ethanol Extract from Hippophae Rhamnoides L. Leaves towards Human Acute Myeloid Leukemia Cells In Vitro

G. T. Zhamanbaeva, M. K. Murzakhmetova, S. T. Tuleukhanov, M. P. Danilenko

    Research output: Contribution to journalArticlepeer-review

    16 Scopus citations

    Abstract

    We studied the effects of ethanol extract from Hippophae rhamnoides L. leaves on the growth and differentiation of human acute myeloid leukemia cells (KG-1a, HL60, and U937). The extract of Hippophae rhamnoides L. leaves inhibited cell growth depending on the cell strain and extract dose. In a high concentration (100 μg/ml), the extract also exhibited a cytotoxic effect on HL60 cells. Hippophae rhamnoides L. leaves extract did not affect cell differentiation and did not modify the differentiating effect of calcitriol, active vitamin D metabolite. Inhibition of cell proliferation was paralleled by paradoxical accumulation of phase S cells (synthetic phase) with a reciprocal decrease in the count of G1 cells (presynthetic phase). The extract in a concentration of 100 μg/ml induced the appearance of cells with a subdiploid DNA content (sub-G1 phase cells), which indicated induction of apoptosis. The antiproliferative effect of Hippophae rhamnoides L. extract on acute myeloid leukemia cells was at least partially determined by activation of the S phase checkpoint, which probably led to deceleration of the cell cycle and apoptosis induction.

    Original languageEnglish
    Pages (from-to)252-255
    Number of pages4
    JournalBulletin of Experimental Biology and Medicine
    Volume158
    Issue number2
    DOIs
    StatePublished - 1 Dec 2014

    Keywords

    • acute myeloid leukemia
    • apoptosis
    • cytotoxicity
    • extract of Hippophae rhamnoides L. leaves
    • proliferation

    ASJC Scopus subject areas

    • General Biochemistry, Genetics and Molecular Biology

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