Application of FTIR microscopy for the characterization of malignancy: H-ras transfected murine fibroblasts as an example

Jagannathan Ramesh, Ahmad Salman, Ziad Hammody, Beny Cohen, Jacob Gopas, Nili Grossman, Shaul Mordechai

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Recently, microscopic FTIR is widely used in the field of biology and medicine. FTIR can detect biomolecular changes in the cells and tissues responsible for various disorders. In this report, we characterize the H-ras transfected fibroblasts and its normal control using microscopic FTIR. The intensity of the normal fibroblasts was higher than that of H-ras transfected fibroblasts. Our studies showed significant differences occur in the concentration of vital metabolites upon transformation. The DNA and carbohydrates level decreased in the transformed cells compared to the controls. A linear correlation could be found between the levels of carbohydrates and phosphate, while the RNA/DNA ratio varied inversely with glucose/phosphate levels.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalJournal of Biochemical and Biophysical Methods
Volume50
Issue number1
DOIs
StatePublished - 4 Dec 2001

Keywords

  • Cancer
  • FTIR microscopy
  • Fibroblasts
  • H-ras
  • Oncogenes

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