Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome

Tal Paz-Aviram; Avital Yahalom; Daniel A. Chamovitz

doi:10.4161/psb.3.6.5434

Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome

Tal Paz-Aviram, Avital Yahalom, Daniel A. Chamovitz

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The roles of individual Eukaryotic translation Initiation Factor 3 (eIF3) subunits are largely unclear. Though some are essential, while others are thought to have regulatory roles. The "e" subunit, also known as Int-6, is a candidate for a regulatory subunit as it is not essential for translation initiation in yeasts. To further elucidate the roles of eIF3e, we have employed an interaction-trap screen using the yeast two-hybrid system. eIF3e interacts in yeast with subunits of the ribosome, COP9 signalosome and 26S proteasome. These interactions mesh well with our recent results which showed that eIF3e is degraded in a CSN-dependent, proteasome-dependent fashion, and inhibits translation when present in excess.

Original language	English
Pages (from-to)	409-411
Number of pages	3
Journal	Plant Signaling and Behavior
Volume	3
Issue number	6
DOIs	https://doi.org/10.4161/psb.3.6.5434
State	Published - 1 Jan 2008
Externally published	Yes

Keywords

COP9 signalosome
Int6
Proteasome
eIF3
eIF3e

ASJC Scopus subject areas

Plant Science

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10.4161/psb.3.6.5434

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title = "Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome",

abstract = "The roles of individual Eukaryotic translation Initiation Factor 3 (eIF3) subunits are largely unclear. Though some are essential, while others are thought to have regulatory roles. The {"}e{"} subunit, also known as Int-6, is a candidate for a regulatory subunit as it is not essential for translation initiation in yeasts. To further elucidate the roles of eIF3e, we have employed an interaction-trap screen using the yeast two-hybrid system. eIF3e interacts in yeast with subunits of the ribosome, COP9 signalosome and 26S proteasome. These interactions mesh well with our recent results which showed that eIF3e is degraded in a CSN-dependent, proteasome-dependent fashion, and inhibits translation when present in excess.",

keywords = "COP9 signalosome, Int6, Proteasome, eIF3, eIF3e",

author = "Tal Paz-Aviram and Avital Yahalom and Chamovitz, {Daniel A.}",

note = "Funding Information: the interaction of eIF3e with degradation of specific cell cynot the proteasome arises from eIF3e being cle regulators, it is also conceivable, that a proteasome substrate.27 Acknowledgements This researchD waos supported by Research Grant Award No. IS-3901-06c from BARD, The United States-Israel Binational Agricultural Research and Development Fund.",

year = "2008",

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doi = "10.4161/psb.3.6.5434",

language = "English",

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journal = "Plant Signaling and Behavior",

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T1 - Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome

AU - Paz-Aviram, Tal

AU - Yahalom, Avital

AU - Chamovitz, Daniel A.

N1 - Funding Information: the interaction of eIF3e with degradation of specific cell cynot the proteasome arises from eIF3e being cle regulators, it is also conceivable, that a proteasome substrate.27 Acknowledgements This researchD waos supported by Research Grant Award No. IS-3901-06c from BARD, The United States-Israel Binational Agricultural Research and Development Fund.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The roles of individual Eukaryotic translation Initiation Factor 3 (eIF3) subunits are largely unclear. Though some are essential, while others are thought to have regulatory roles. The "e" subunit, also known as Int-6, is a candidate for a regulatory subunit as it is not essential for translation initiation in yeasts. To further elucidate the roles of eIF3e, we have employed an interaction-trap screen using the yeast two-hybrid system. eIF3e interacts in yeast with subunits of the ribosome, COP9 signalosome and 26S proteasome. These interactions mesh well with our recent results which showed that eIF3e is degraded in a CSN-dependent, proteasome-dependent fashion, and inhibits translation when present in excess.

AB - The roles of individual Eukaryotic translation Initiation Factor 3 (eIF3) subunits are largely unclear. Though some are essential, while others are thought to have regulatory roles. The "e" subunit, also known as Int-6, is a candidate for a regulatory subunit as it is not essential for translation initiation in yeasts. To further elucidate the roles of eIF3e, we have employed an interaction-trap screen using the yeast two-hybrid system. eIF3e interacts in yeast with subunits of the ribosome, COP9 signalosome and 26S proteasome. These interactions mesh well with our recent results which showed that eIF3e is degraded in a CSN-dependent, proteasome-dependent fashion, and inhibits translation when present in excess.

KW - COP9 signalosome

KW - Int6

KW - Proteasome

KW - eIF3

KW - eIF3e

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U2 - 10.4161/psb.3.6.5434

DO - 10.4161/psb.3.6.5434

M3 - Article

AN - SCOPUS:44649197065

SN - 1559-2316

VL - 3

SP - 409

EP - 411

JO - Plant Signaling and Behavior

JF - Plant Signaling and Behavior

IS - 6

ER -

Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome

Abstract

Keywords

ASJC Scopus subject areas

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