TY - JOUR
T1 - Ascorbate peroxidase gene family in tomato
T2 - Its identification and characterization
AU - Najami, Naim
AU - Janda, Tibor
AU - Barriah, Waseim
AU - Kayam, Galya
AU - Tal, Moshe
AU - Guy, Micha
AU - Volokita, Micha
N1 - Funding Information:
Acknowledgments We thank Prof. L. Reinhold for critical reading of the manuscript and many useful comments. The study was supported by Dr. Herman Kessel Research Fund, in the memory of Mr. C. J. J. Van Kensbury.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - The antioxidative response, where ascorbate peroxidase (APX) is a key enzyme, is an integral part of the plant tolerance response to environmental stresses. As a first step towards the study of the physiological role and the regulation of the members of the Apx gene family, the orthologs of the stress-sensitive cultivated tomato Solanum lycopersicum cv. M82 (Slm) and of the wild salt-tolerant species S. pennellii acc. Atico (Spa) were identified by utilizing the tomato EST database, and characterized. A redundant list of 16 virtual Apx transcripts and four singleton ESTs was shown to correspond to seven genuine Apx genes. The complete tomato Apx gene family is comprised of genes encoding three cytosolic, two peroxisomal, and two chloroplastic APXs. These genes attained differential regulatory patterns in various Slm organs. More detailed study of Apx1 and Apx2 genes, that are the products of a recent gene duplication event, shows that they have already attained differential regulation within and between Slm and Spa under control and stress conditions. It is also suggested that due to lineage-specific gene duplication and lose events, intricate phylogenetic relationships exist among the members of the Apx gene families.
AB - The antioxidative response, where ascorbate peroxidase (APX) is a key enzyme, is an integral part of the plant tolerance response to environmental stresses. As a first step towards the study of the physiological role and the regulation of the members of the Apx gene family, the orthologs of the stress-sensitive cultivated tomato Solanum lycopersicum cv. M82 (Slm) and of the wild salt-tolerant species S. pennellii acc. Atico (Spa) were identified by utilizing the tomato EST database, and characterized. A redundant list of 16 virtual Apx transcripts and four singleton ESTs was shown to correspond to seven genuine Apx genes. The complete tomato Apx gene family is comprised of genes encoding three cytosolic, two peroxisomal, and two chloroplastic APXs. These genes attained differential regulatory patterns in various Slm organs. More detailed study of Apx1 and Apx2 genes, that are the products of a recent gene duplication event, shows that they have already attained differential regulation within and between Slm and Spa under control and stress conditions. It is also suggested that due to lineage-specific gene duplication and lose events, intricate phylogenetic relationships exist among the members of the Apx gene families.
KW - Ascorbate peroxidase
KW - Gene duplication
KW - Gene expression
KW - Gene family
KW - Tomato
UR - http://www.scopus.com/inward/record.url?scp=38349042069&partnerID=8YFLogxK
U2 - 10.1007/s00438-007-0305-2
DO - 10.1007/s00438-007-0305-2
M3 - Article
AN - SCOPUS:38349042069
SN - 1617-4615
VL - 279
SP - 171
EP - 182
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 2
ER -