Asgard ESCRT-III and VPS4 reveal conserved chromatin binding properties of the ESCRT machinery

Dikla Nachmias, Nataly Melnikov, Alvah Zorea, Maya Sharon, Reut Yemini, Yasmin De-picchoto, Ioannis Tsirkas, Amir Aharoni, Bela Frohn, Petra Schwille, Raz Zarivach, Itzhak Mizrahi, Natalie Elia

Research output: Contribution to journalArticlepeer-review

Abstract

The archaeal Asgard superphylum currently stands as the most promising prokaryotic candidate, from which eukaryotic cells emerged. This unique superphylum encodes for eukaryotic signature proteins (ESP) that could shed light on the origin of eukaryotes, but the properties and function of these proteins is largely unresolved. Here, we set to understand the function of an Asgard archaeal protein family, namely the ESCRT machinery, that is conserved across all domains of life and executes basic cellular eukaryotic functions, including membrane constriction during cell division. We find that ESCRT proteins encoded in Loki archaea, express in mammalian and yeast cells, and that the Loki ESCRT-III protein, CHMP4-7, resides in the eukaryotic nucleus in both organisms. Moreover, Loki ESCRT-III proteins associated with chromatin, recruited their AAA-ATPase VPS4 counterpart to organize in discrete foci in the mammalian nucleus, and directly bind DNA. The human ESCRT-III protein, CHMP1B, exhibited similar nuclear properties and recruited both human and Asgard VPS4s to nuclear foci, indicating interspecies interactions. Mutation analysis revealed a role for the N terminal region of ESCRT-III in mediating these phenotypes in both human and Asgard ESCRTs. These findings suggest that ESCRT proteins hold chromatin binding properties that were highly preserved through the billion years of evolution separating Asgard archaea and humans. The conserved chromatin binding properties of the ESCRT membrane remodeling machinery, reported here, may have important implications for the origin of eukaryogenesis.

Original languageEnglish
Pages (from-to)117-129
Number of pages13
JournalISME Journal
Volume17
Issue number1
DOIs
StatePublished - 1 Jan 2023

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Fingerprint

Dive into the research topics of 'Asgard ESCRT-III and VPS4 reveal conserved chromatin binding properties of the ESCRT machinery'. Together they form a unique fingerprint.

Cite this