Assay of Ca2+ transport by VDAC1 reconstituted into liposomes

Danya Ben-Hail, Varda Shoshan-Barmatz

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Ca2+ permeability mediated by voltage-dependent anion-selective channel protein 1 (VDAC1) can be tested by reconstitution of purified VDAC1 into liposomes. Here, we describe a setup for this membranal system, which has been used to study the transport activity of various transporters, including VDAC1, and allows detection of the passage of molecules across the lipid bilayer. Despite the disadvantage of needing radiolabeled molecules, this system is highly desirable when the transport properties of noncharged molecules and/or active transporters are studied.

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalCold Spring Harbor Protocols
Volume2014
Issue number2
DOIs
StatePublished - 1 Feb 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)

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