Assessment of genetic stability in micro propagated population of sugarcane variety CoS 07250 through SSR markers

A. K. Tiwari; N. Mishra; S. Tripathi; M. Lal; R. K. Singh; M. L. Sharma

Assessment of genetic stability in micro propagated population of sugarcane variety CoS 07250 through SSR markers

A. K. Tiwari, N. Mishra, S. Tripathi, M. Lal, R. K. Singh, M. L. Sharma

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Newly released variety of sugarcane (CoS 07250) was micropropogated using shoot tip culture technique. Genetic purity was assessed in regenerated plants using Simple Sequence Repeat (SSR) markers. Eleven SSR primers were used to amplify the genomic DNA extracted from fresh leaf samples of in vitro raised plantlets after proper hardening along with the donar. A total of 1158 bands appeared out of which 121 bands were found polymorphic with an average of 11 bands per primer. The genetic similarity ranged between 0.85 and 1.00. The cluster analysis indicated a close relationship between regenerated plants and donar. The results indicated a high degree of genetic stability among the micropropogated plants. Thus the micropropagation protocol can be used to produce true to the type plants in sugarcane. It is suggested that plantlets multiplied through tissue culture should be tested through molecular tools for clonal stability before certification and distribution.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Vegetos
Volume	24
Issue number	2
State	Published - 1 Dec 2011
Externally published	Yes

Keywords

Genetic fidelity
PCR
References
SSR marker
Sugarcane

ASJC Scopus subject areas

Plant Science

Cite this

@article{464a543c079445528bdabeaf2b1f9933,

title = "Assessment of genetic stability in micro propagated population of sugarcane variety CoS 07250 through SSR markers",

abstract = "Newly released variety of sugarcane (CoS 07250) was micropropogated using shoot tip culture technique. Genetic purity was assessed in regenerated plants using Simple Sequence Repeat (SSR) markers. Eleven SSR primers were used to amplify the genomic DNA extracted from fresh leaf samples of in vitro raised plantlets after proper hardening along with the donar. A total of 1158 bands appeared out of which 121 bands were found polymorphic with an average of 11 bands per primer. The genetic similarity ranged between 0.85 and 1.00. The cluster analysis indicated a close relationship between regenerated plants and donar. The results indicated a high degree of genetic stability among the micropropogated plants. Thus the micropropagation protocol can be used to produce true to the type plants in sugarcane. It is suggested that plantlets multiplied through tissue culture should be tested through molecular tools for clonal stability before certification and distribution.",

keywords = "Genetic fidelity, PCR, References, SSR marker, Sugarcane",

author = "Tiwari, {A. K.} and N. Mishra and S. Tripathi and M. Lal and Singh, {R. K.} and Sharma, {M. L.}",

year = "2011",

month = dec,

day = "1",

language = "English",

volume = "24",

pages = "75--81",

journal = "Vegetos",

issn = "0970-4078",

publisher = "Society for Plant Research, Department of Botany, Bareilly College",

number = "2",

}

TY - JOUR

T1 - Assessment of genetic stability in micro propagated population of sugarcane variety CoS 07250 through SSR markers

AU - Tiwari, A. K.

AU - Mishra, N.

AU - Tripathi, S.

AU - Lal, M.

AU - Singh, R. K.

AU - Sharma, M. L.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Newly released variety of sugarcane (CoS 07250) was micropropogated using shoot tip culture technique. Genetic purity was assessed in regenerated plants using Simple Sequence Repeat (SSR) markers. Eleven SSR primers were used to amplify the genomic DNA extracted from fresh leaf samples of in vitro raised plantlets after proper hardening along with the donar. A total of 1158 bands appeared out of which 121 bands were found polymorphic with an average of 11 bands per primer. The genetic similarity ranged between 0.85 and 1.00. The cluster analysis indicated a close relationship between regenerated plants and donar. The results indicated a high degree of genetic stability among the micropropogated plants. Thus the micropropagation protocol can be used to produce true to the type plants in sugarcane. It is suggested that plantlets multiplied through tissue culture should be tested through molecular tools for clonal stability before certification and distribution.

AB - Newly released variety of sugarcane (CoS 07250) was micropropogated using shoot tip culture technique. Genetic purity was assessed in regenerated plants using Simple Sequence Repeat (SSR) markers. Eleven SSR primers were used to amplify the genomic DNA extracted from fresh leaf samples of in vitro raised plantlets after proper hardening along with the donar. A total of 1158 bands appeared out of which 121 bands were found polymorphic with an average of 11 bands per primer. The genetic similarity ranged between 0.85 and 1.00. The cluster analysis indicated a close relationship between regenerated plants and donar. The results indicated a high degree of genetic stability among the micropropogated plants. Thus the micropropagation protocol can be used to produce true to the type plants in sugarcane. It is suggested that plantlets multiplied through tissue culture should be tested through molecular tools for clonal stability before certification and distribution.

KW - Genetic fidelity

KW - PCR

KW - References

KW - SSR marker

KW - Sugarcane

UR - http://www.scopus.com/inward/record.url?scp=84863162055&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84863162055

SN - 0970-4078

VL - 24

SP - 75

EP - 81

JO - Vegetos

JF - Vegetos

IS - 2

ER -

Assessment of genetic stability in micro propagated population of sugarcane variety CoS 07250 through SSR markers

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this