AtnMat2, a nuclear-encoded maturase required for splicing of group-II introns in Arabidopsis mitochondria

Ido Keren, Ayenachew Bezawork-Geleta, Max Kolton, Inbar Maayan, Eduard Belausov, Maggie Levy, Anahit Mett, David Gidoni, Felix Shaya, Oren Ostersetzer-Biran

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Mitochondria (mt) in plants house about 20 group-II introns, which lie within protein-coding genes required in both organellar genome expression and respiration activities. While in nonplant systems the splicing of group-II introns is mediated by proteins encoded within the introns themselves (known as "maturases"), only a single maturase ORF (matR) has retained in the mitochondrial genomes in plants; however, its putative role(s) in the splicing of organellar introns is yet to be established. Clues to other proteins are scarce, but these are likely encoded within the nucleus as there are no obvious candidates among the remaining ORFs within the mtDNA. Intriguingly, higher plants genomes contain four maturase-related genes, which exist in the nucleus as self-standing ORFs, out of the context of their evolutionary-related group-II introns "hosts." These are all predicted to reside within mitochondria and may therefore act "in-trans" in the splicing of organellar-encoded introns. Here, we analyzed the intracellular locations of the four nuclear-encoded maturases in Arabidopsis and established the roles of one of these genes, At5g46920 (AtnMat2), in the splicing of several mitochondrial introns, including the single intron within cox2, nad1 intron2, and nad7 intron2.

Original languageEnglish
Pages (from-to)2299-2311
Number of pages13
JournalRNA
Volume15
Issue number12
DOIs
StatePublished - 1 Dec 2009
Externally publishedYes

Keywords

  • Group-II introns
  • Maturases
  • Mitochondria
  • Plant
  • Splicing

ASJC Scopus subject areas

  • Molecular Biology

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