ATP-Decorated Mesoporous Silica for Biomineralization of Calcium Carbonate and P2 Purinergic Receptor-Mediated Antitumor Activity against Aggressive Lymphoma

Prateek Srivastava, Sumit Kumar Hira, Divesh Narayan Srivastava, Vivek Kumar Singh, Uttam Gupta, Ranjeet Singh, Ram Adhar Singh, Partha Pratim Manna

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Adenosine triphosphate (ATP) is an important transmitter that mediates various biological effects via purinergic receptors (P2 receptors) in cancer. We investigated the antitumor activity of ATP-decorated and doxorubicin (DOX)-loaded mesoporous silica with biomineralization of calcium carbonate against a highly aggressive and metastatic murine lymphoma called Dalton's lymphoma (DL). Our results suggest that this nanocomposite has unique effects with respect to the morphology and properties of calcium carbonate on the surface of the nanoparticle. DOX in the nanoparticles was prevented from quick release via the interactions of the phosphate group present on ATP and calcium carbonate. This construct is significantly tumoricidal against parental and DOX-resistant DL cells and is thus a promising candidate for applications in drug delivery. The composite nanomaterial has excellent biocompatibility with higher uptake and acts via the participation of the purinergic receptor P2X7. The nanocomposite induces significantly higher apoptosis in tumor cells compared with DOX alone. Treatment of DL-bearing mice with the construct significantly reduces tumor burden, in addition to augmenting the lifespan of tumor-bearing mice as demonstrated by a sustained healthy life of the animals and improved histopathological parameters.

Original languageEnglish
Pages (from-to)6917-6929
Number of pages13
JournalACS applied materials & interfaces
Volume10
Issue number8
DOIs
StatePublished - 28 Feb 2018
Externally publishedYes

Keywords

  • ATP
  • P2X7
  • apoptosis
  • biomineralization
  • doxorubicin
  • lymphoma
  • mesoporous silica

ASJC Scopus subject areas

  • General Materials Science

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