TY - JOUR
T1 - ATP Synthesis and Hydrolysis in Chloroplast Membranes
T2 - Differential Inhibition by Antibodies to Chloroplast Coupling Factor 1
AU - SHOSHAN, Varda
AU - SHAVIT, Noun
PY - 1979/1/1
Y1 - 1979/1/1
N2 - Divalent antibodies against chloroplast coupling factor 1 inhibited the factor ATPase, ATP synthesis, hydrolysis and Pi‐ATP exchange in chloroplasts. These antibodies also inhibited coupled electron flow rates but not the basal or uncoupled rates. Several types of non‐precipitating, modified antibodies prepared from the original antibody preparation strongly inhibited the ATPase and Pi‐ATP exchange reaction but had little effect on ATP formation. It is suggested that the inhibition of ATP synthesis by the divalent antibodies is probably due to an indirect blocking of the active site, while the inhibition of ATP‐utilizing reactions by the modified antibodies is related to their effect on the transfer of ATP from a non‐catalytic to a catalytic site on coupling factor 1, via an energy‐dependent conformational change.
AB - Divalent antibodies against chloroplast coupling factor 1 inhibited the factor ATPase, ATP synthesis, hydrolysis and Pi‐ATP exchange in chloroplasts. These antibodies also inhibited coupled electron flow rates but not the basal or uncoupled rates. Several types of non‐precipitating, modified antibodies prepared from the original antibody preparation strongly inhibited the ATPase and Pi‐ATP exchange reaction but had little effect on ATP formation. It is suggested that the inhibition of ATP synthesis by the divalent antibodies is probably due to an indirect blocking of the active site, while the inhibition of ATP‐utilizing reactions by the modified antibodies is related to their effect on the transfer of ATP from a non‐catalytic to a catalytic site on coupling factor 1, via an energy‐dependent conformational change.
UR - http://www.scopus.com/inward/record.url?scp=0018790857&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1979.tb12874.x
DO - 10.1111/j.1432-1033.1979.tb12874.x
M3 - Article
C2 - 155523
AN - SCOPUS:0018790857
SN - 0014-2956
VL - 94
SP - 87
EP - 92
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -