Autoantibodies Profile in the Sera of Patients with Sjogren's Syndrome: The ANA Evaluation - A Homogeneous, Multiplexed System

Boris Gilburd, Mahmoud Abu-Shakra, Yehuda Shoenfeld, Andrea Giordano, Elena Bartoloni Bocci, Francesco Delle Monache, Roberto Gerli

    Research output: Contribution to journalArticlepeer-review

    57 Scopus citations

    Abstract

    Background: Flow-based, multiplex bead arrays (MBA) have been developed for a variety of applications including the detection of antibodies to extractable nuclear antigens (ENA). It offers a rapid and sensitive method to assess multiple analyses in a single tube/well. Purpose: To evaluate the Athena Multi-Lyte ANA Test System utilizes Luminex Corporation's MBA technology for the detection of antinuclear antibodies (ANA) and ENA antibodies in the sera of patients with Sjogren's syndrome (SS). Methods: MBA assay was used to detect ANA and ENA antibodies in the sera of 37 patients with SS and 96 sera from healthy subjects. Results: All patients were women. Their mean age was 48.7 years and the mean disease duration was 7.27 years. ANA was found in 3 (3%) sera of healthy subjects by the AtheNA system and in 2 (2%) sera by the ELISA kit. A 99% concordance between the 2 assays was found. A 94.6% concordance between the 2 assays was found by testing the sera of patients with SS for ANA. By the AtheNA system, none of the sera of 37 patients with SS had autoantibodies reacting with Sm, Jo-1, dsDNA or histones. Anti-RNP antibody was found in 5.4% of the sera and 2.7% of the sera reacted with Scl-70 and histones. Anti-SS/A and anti-SS/B were identified in 84 and 76% of the sera, respectively. Conclusion: The AtheNa Multi-Lyte ANA Test System offers a sensitive and specific result for the detection of ANA and ENA antibodies in the sera of patients with SS.

    Original languageEnglish
    Pages (from-to)53-56
    Number of pages4
    JournalClinical and Developmental Immunology
    Volume11
    Issue number1
    DOIs
    StatePublished - 1 Mar 2004

    Keywords

    • Autoantibodies
    • Extractable nuclear antigens (ENA)
    • Multiplex bead arrays
    • Sjogren's syndrome

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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