Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer

Sebastian Isbaner; Narain Karedla; Izabela Kaminska; Daja Ruhlandt; Mario Raab; Johann Bohlen; Alexey Chizhik; Ingo Gregor; Philip Tinnefeld; Jörg Enderlein; Roman Tsukanov

doi:10.1021/acs.nanolett.8b00425

Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer

Sebastian Isbaner, Narain Karedla, Izabela Kaminska, Daja Ruhlandt, Mario Raab, Johann Bohlen, Alexey Chizhik, Ingo Gregor, Philip Tinnefeld, Jörg Enderlein, Roman Tsukanov

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule metal-induced energy transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm. smMIET relies only on fluorescence lifetime measurements and does not require additional complex optical setups.

Original language	English
Pages (from-to)	2616-2622
Number of pages	7
Journal	Nano Letters
Volume	18
Issue number	4
DOIs	https://doi.org/10.1021/acs.nanolett.8b00425
State	Published - 11 Apr 2018
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Access to Document

10.1021/acs.nanolett.8b00425

Cite this

@article{71fdec9d0af145df87a50035f4ce6911,

title = "Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer",

abstract = "Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule metal-induced energy transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm. smMIET relies only on fluorescence lifetime measurements and does not require additional complex optical setups.",

author = "Sebastian Isbaner and Narain Karedla and Izabela Kaminska and Daja Ruhlandt and Mario Raab and Johann Bohlen and Alexey Chizhik and Ingo Gregor and Philip Tinnefeld and J{\"o}rg Enderlein and Roman Tsukanov",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = apr,

day = "11",

doi = "10.1021/acs.nanolett.8b00425",

language = "English",

volume = "18",

pages = "2616--2622",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer

AU - Isbaner, Sebastian

AU - Karedla, Narain

AU - Kaminska, Izabela

AU - Ruhlandt, Daja

AU - Raab, Mario

AU - Bohlen, Johann

AU - Chizhik, Alexey

AU - Gregor, Ingo

AU - Tinnefeld, Philip

AU - Enderlein, Jörg

AU - Tsukanov, Roman

PY - 2018/4/11

Y1 - 2018/4/11

N2 - Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule metal-induced energy transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm. smMIET relies only on fluorescence lifetime measurements and does not require additional complex optical setups.

AB - Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule metal-induced energy transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm. smMIET relies only on fluorescence lifetime measurements and does not require additional complex optical setups.

UR - http://www.scopus.com/inward/record.url?scp=85045224051&partnerID=8YFLogxK

U2 - 10.1021/acs.nanolett.8b00425

DO - 10.1021/acs.nanolett.8b00425

M3 - Article

C2 - 29562123

AN - SCOPUS:85045224051

SN - 1530-6984

VL - 18

SP - 2616

EP - 2622

JO - Nano Letters

JF - Nano Letters

IS - 4

ER -

Axial Colocalization of Single Molecules with Nanometer Accuracy Using Metal-Induced Energy Transfer

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this