@article{c779857af2ed49d88b8a9017593ad748,
title = "Baculovirus transit through insect cell membranes: A mechanistic approach",
abstract = "Baculovirus systems are used for various purposes, but the kinetics of the infection process is not fully understood yet. We investigated the dynamics of virion movement from a medium toward the interior of insect cells and established a mechanistic model that shows an excellent fit to experimental results. It also makes possible a description of the viral dynamics on the cell surface. A novel measurement method was used to distinguish between infected cells that carry virions on their surfaces, cells that carry virions in their interior, and those carrying virions both inside and on their surface. The maximum number of virions carried by a cell: 55 viruses/cell, and the time required for viral internalization, 0.8h, are reported. This information is particularly useful for assessing the infection efficacy and the required number of virions needed to infect a given cell population. Although our model specifically concerns the infection process of Sf9 insect cells by baculovirus, it describes general features of viral infection. Some of the model features may eventually be applicable in the studies towards palliation of the COVID-19 outbreak.",
keywords = "Baculovirus, Infection Simulation, Infection kinetics, Mathematical model",
author = "Youhong Zhang and Giora Enden and Wei Wei and Feng Zhou and Jie Chen and Merchuk, {Jose C.}",
note = "Funding Information: This research was financially supported by the National Natural Science Foundation of China (20876120 and 20910102037) and the Nature Science Foundation of Hubei, China (2013CFA101). We thank Professor Qi Yipeng of Wuhan University, China, for kindly providing wt AcMNPV. Y. H. Z. conceived the project and led the research. G. E. and J. C. M. analyzed the data, developed the models and performed the calculations. W. W. and F. Z. performed all experiments. J. C. guided the performing of the FCM detections. Y. H. Z. G. E. and J. C. M. discussed the results and contributed to the final manuscript. This article does not contain any studies with human participants or animals performed by any of the authors. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Funding Information: This research was financially supported by the National Natural Science Foundation of China ( 20876120 and 20910102037 ) and the Nature Science Foundation of Hubei , China ( 2013CFA101 ). We thank Professor Qi Yipeng of Wuhan University, China, for kindly providing wt AcMNPV. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = sep,
day = "21",
doi = "10.1016/j.ces.2020.115727",
language = "English",
volume = "223",
journal = "Chemical Engineering Science",
issn = "0009-2509",
publisher = "Elsevier BV",
}