Bax and Bif-1 proteins interact on Bilayer Lipid Membrane and form pore

Rajeev Gupta, Subhendu Ghosh

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Bax and Bax interacting factor-1(Bif-1) are cytosolic proteins, which translocate towards mitochondria during mitochondria-mediated apoptosis. Bif-1 has been identified to co-immunoprecipitate with Bax in apoptotic cells. We have studied the interaction of Bax and Bif-1 on Bilayer Lipid Membrane (BLM) through electrophysiological experiments. It has been observed that Bax-Bif-1 equimolar mixture can form a pore. The pore conductance is in the range of 4.96-5.41 nS. It also displays a sub-state with a conductance of 2.6 nS. No pore activity is observed on BLM when monomeric Bax and Bif-1 proteins are tested independently. The above-mentioned pore forming activity could be relevant in mitochondria-mediated apoptosis.

Original languageEnglish
Pages (from-to)751-755
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume463
Issue number4
DOIs
StatePublished - 13 Jul 2015
Externally publishedYes

Keywords

  • Bax
  • Bif-1
  • Bilayer electrophysiology
  • Mitochondria-mediated apoptosis
  • Pore

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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