Beyond the cytoplasm of Escherichia coli: Localizing recombinant proteins where you want them

Jason T. Boock; Dujduan Waraho-Zhmayev; Dario Mizrachi; Matthew P. DeLisa

doi:10.1007/978-1-4939-2205-5_5

Beyond the cytoplasm of Escherichia coli: Localizing recombinant proteins where you want them

Jason T. Boock, Dujduan Waraho-Zhmayev, Dario Mizrachi, Matthew P. DeLisa

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Recombinant protein expression in Escherichia coli represents a cornerstone of the biotechnology enterprise. While cytoplasmic expression in this host has received the most attention, achieving substantial yields of correctly folded proteins in this compartment can sometimes be met with difficulties. These issues can often be overcome by targeting protein expression to extracytoplasmic compartments (e.g., membrane, periplasm) or to the culture medium. This chapter discusses various strategies for exporting proteins out of the cytoplasm as well as tools for monitoring and optimizing these different export mechanisms.

Original language	English
Pages (from-to)	79-97
Number of pages	19
Journal	Methods in Molecular Biology
Volume	1258
DOIs	https://doi.org/10.1007/978-1-4939-2205-5_5
State	Published - 1 Jan 2015
Externally published	Yes

Keywords

Cell factories
Escherichia coli
Extracellular protein production
General secretory pathway
Membrane protein expression
Twin-arginine translocation
YebF

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2205-5_5

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abstract = "Recombinant protein expression in Escherichia coli represents a cornerstone of the biotechnology enterprise. While cytoplasmic expression in this host has received the most attention, achieving substantial yields of correctly folded proteins in this compartment can sometimes be met with difficulties. These issues can often be overcome by targeting protein expression to extracytoplasmic compartments (e.g., membrane, periplasm) or to the culture medium. This chapter discusses various strategies for exporting proteins out of the cytoplasm as well as tools for monitoring and optimizing these different export mechanisms.",

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Beyond the cytoplasm of Escherichia coli: Localizing recombinant proteins where you want them

Abstract

Keywords

ASJC Scopus subject areas

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