Binding assay and preliminary X-ray crystallographic analysis of ACTIBIND, a protein with anticarcinogenic and antiangiogenic activities

Marina De Leeuw, Levava Roiz, Patricia Smirnoff, Betty Schwartz, Oded Shoseyov, Orna Almog

    Research output: Contribution to journalArticlepeer-review

    8 Scopus citations

    Abstract

    ACTIBIND is a T2 RNase extracellular glycoprotein produced by the mould Aspergillus niger B1 (CMI CC 324626) that possesses anticarcinogenic and antiangiogenic activities. ACTIBIND was found to be an actin-binding protein that interacts with rabbit muscle actin in a 1:2 molar ratio (ACTIBIND:actin) with a binding constant of 16.17 × 104 M -1. Autoclave-treated ACTIBIND (EI-ACTIBIND) lost its RNase activity, but its actin-binding ability was conserved. ACTIBIND crystals were grown using 20% PEG 3350, 0.2 M ammonium dihydrogen phosphate solution at room temperature (293 K). One to four single crystals appeared in each droplet within a few days and grew to approximate dimensions of 0.5 x 0.5 x 0.5 mm after about two weeks. Diffraction studies of these crystals at low temperature (100 K) indicated that they belong to the P3121 space group, with unit-cell parameters a = 78, b = 78, c = 104 Å.

    Original languageEnglish
    Pages (from-to)716-719
    Number of pages4
    JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
    Volume63
    Issue number8
    DOIs
    StatePublished - 28 Jul 2007

    Keywords

    • ACTIBIND
    • Antiangiogens
    • Anticarcinogens
    • RNase T2

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Genetics
    • Condensed Matter Physics

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