Binding of Nicotiana nuclear proteins to the subterminal regions of the Ac transposable element

Avraham A. Levy, Marcelo Fridlender, Uri Hanania, Eitan Rubin, Yaron Sitrit

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Specific binding of Nicotiana nuclear protein(s) to subterminal regions of the Ac transposable element was detected using gel mobility shift assays. A sequence motif (GGTAAA) repeated in both terminal regions of Ac, was identified as the protein binding site. Mutation of two nucleotides in this motif was sufficient to abolish binding. Based on a series of competition assays, it is deduced that there is cooperative binding between two repeats, each similar to the GGTAAA motif. The binding protein is probably similar to a previously characterized maize protein which binds to a GGTAAA-containing motif located in the ends of Mutator. Moreover, we show that DNA from Ds1 competes for protein binding to Ac termini, and we show, by sequence analysis, that GGTAAA binding sites are present in the terminal region of Tgm1, Tpn1, En/Spm, Tam3 and Ds1-like elements. This suggests that the binding protein(s) might be involved in the transposition process.

Original languageEnglish
Pages (from-to)436-441
Number of pages6
JournalMolecular and General Genetics
Volume251
Issue number4
DOIs
StatePublished - 1 Jan 1996
Externally publishedYes

Keywords

  • Activator (Ac)
  • DNA-binding proteins
  • Host factor
  • Nicotiana
  • Transposition

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Binding of Nicotiana nuclear proteins to the subterminal regions of the Ac transposable element'. Together they form a unique fingerprint.

Cite this