Bio-Functionalized Silver Nanoparticles: a Novel Colorimetric Probe for Cysteine Detection

Hemant P. Borase, Chandrashekhar D. Patil, Rahul B. Salunkhe, Rahul K. Suryawanshi, Beom S. Kim, Vishwas A. Bapat, Satish V. Patil

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Chemical interactions between nanoparticles and biomolecules are vital for applying nanoparticles in medicine and life science. Development of sensitive, rapid, low-cost, and eco-friendly sensors for the detection of molecules acting as disease indicator is need of an hour. In the present investigation, a green trend for silver nanoparticle synthesis was followed using leaf extract of Calotropis procera. Silver nanoparticles exhibited surface plasmon absorption peak at 421 nm, spherical shape with average size of 10 nm, and zeta potential of −22.4 mV. The as-synthesized silver nanoparticles were used for selective and sensitive detection of cysteine. Cysteine induces aggregation in stable silver nanoparticles owing to selective and strong interaction of –SH group of cysteine with silver nanoparticle surface. Cysteine-induced silver nanoparticle aggregation can be observed visually by change in color of silver nanoparticles from yellow to pink. Cysteine concentration was estimated colorimetrically by measuring absorption at surface plasmon wavelength. Limit of detection for cysteine using silver nanoparticles is ultralow, i.e., 100 nM. The mechanistic insight into cysteine detection by silver nanoparticles was investigated using FT-IR, TEM, DLS, and TLC analysis. Proposed method can be applied for the detection of cysteine in blood plasma and may give rise to a new insight into development of eco-friendly fabricated nanodiagnostic device in future.

Original languageEnglish
Pages (from-to)3479-3493
Number of pages15
JournalApplied Biochemistry and Biotechnology
Issue number7
StatePublished - 1 Apr 2015
Externally publishedYes


  • Blood plasma
  • Colorimetric sensor
  • Cysteine
  • FT-IR
  • Silver nanoparticles

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


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