Biochemical analysis and electron microscopic study on intracellular virions in NIH/3T3 mouse cells chronically infected with Moloney murine leukaemia virus: Effect of interferon

M. Aboud, R. Shoor, S. Bari, Y. Hasan, R. Shurtz, Z. Malik, S. Salzberg

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Radioactively labelled virus particles of intracellular origin were isolated from the cytoplasmic fraction of disrupted NIH/3T3 cells chronically infected with Moloney murine leukaemia virus [NIH/3T3 (MLV)]. Interferon (IFN) treatment for 48 h, which arrested more than 90% of virus release, resulted in a remarkable accumulation of these intracellular virions. However, no major effect of such treatment was apparent on their structural properties. Transmission electron microscopic examination revealed that these intracellular virions were located within cytoplasmic vacuoles. IFN treatment resulted in a considerable increase in the number of virus-containing vacuoles, as well as the total number of vacuolar virions. It seems that IFN inhibits the final release of vacuolar virions from the cells, thus leading to their intracellular accumulation.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalUnknown Journal
Volume62
Issue number2
DOIs
StatePublished - 1 Jan 1982

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of 'Biochemical analysis and electron microscopic study on intracellular virions in NIH/3T3 mouse cells chronically infected with Moloney murine leukaemia virus: Effect of interferon'. Together they form a unique fingerprint.

Cite this